Melato M, Mustac E, Valkovic T, Bottin C, Sasso F, Jonjic N
Department of Biomedical Science, Medical Faculty, University of Rijeka, Croatia.
Pathol Res Pract. 1998;194(9):603-8. doi: 10.1016/S0344-0338(98)80094-0.
Peanut agglutinin (PNA) lectin-binding site patterns in primary invasive breast ductal not otherwise specified (NOS) carcinomas are related to aggressiveness of the tumor. The present study was designed to compare the expression of PNA-binding sites in the primary tumor and in local lymph node metastases. The expression of lectin-binding sites was studied using the avidin-biotin complex/immunoperoxidase technique and analyzed in relation to age of the patient and size of the breast cancer. Breast cancers and their metastases showed negativity or positivity, the latter being divided into "apical" and "non-apical" (i.e. membrane and/or cytoplasmic) depending on the main localization of staining in tumor cells. No correlation was found between primary tumors and metastases as regards PNA-binding patterns, which confirms the opinion that advanced primary tumors are polyclonal and that selected subclones of malignant cells give rise to metastases. Furthermore, the fact that primary tumors with PNA non-apical expression, a feature related to aggressiveness and poor differentiation, may have lymph node metastases with apical expression, suggests that this pattern, although no longer evident in the primary tumor, is involved in the process of cell metastasis.
未另作说明（NOS）的原发性浸润性乳腺导管癌中花生凝集素（PNA）凝集素结合位点模式与肿瘤侵袭性相关。本研究旨在比较原发性肿瘤和局部淋巴结转移灶中PNA结合位点的表达情况。采用抗生物素蛋白-生物素复合物/免疫过氧化物酶技术研究凝集素结合位点的表达，并分析其与患者年龄和乳腺癌大小的关系。乳腺癌及其转移灶表现为阴性或阳性，后者根据肿瘤细胞染色的主要定位分为“顶端”和“非顶端”（即膜和/或细胞质）。原发性肿瘤和转移灶在PNA结合模式方面未发现相关性，这证实了晚期原发性肿瘤是多克隆性的这一观点，即恶性细胞的特定亚克隆会导致转移。此外，具有PNA非顶端表达（一种与侵袭性和低分化相关的特征）的原发性肿瘤可能有顶端表达的淋巴结转移，这表明这种模式虽然在原发性肿瘤中不再明显，但参与了细胞转移过程。
