基质孕酮受体介导孕酮对雌激素诱导的子宫上皮细胞脱氧核糖核酸合成的抑制作用。

Stromal progesterone receptors mediate the inhibitory effects of progesterone on estrogen-induced uterine epithelial cell deoxyribonucleic acid synthesis.

作者信息

Kurita T, Young P, Brody J R, Lydon J P, O'Malley B W, Cunha G R

机构信息

Department of Anatomy, University of California, San Francisco 94143, USA.

出版信息

Endocrinology. 1998 Nov;139(11):4708-13. doi: 10.1210/endo.139.11.6317.

DOI:10.1210/endo.139.11.6317

PMID:9794483

Abstract

The role of epithelial and stromal progesterone (P) receptors (PR) in the regulation of uterine epithelial DNA synthesis by P was investigated by analyzing the four types of tissue recombinants prepared with uterine stroma (S) and epithelium (E) from wild-type (wt) and PR knockout (PRKO) mice: wt-S + wt-E, PRKO-S + PRKO-E, wt-S + PRKO-E, and PRKO-S + wt-E. 17-Beta estradiol (E2) stimulated DNA synthesis in all four types of tissue recombinants. On the other hand, P inhibited E2-induced DNA synthesis only in tissue recombinants prepared with wild-type (PR-positive) stroma (wt-S + wt-E or wt-S + PRKO-E) but not knockout (PR-negative) stroma (PRKO-S + wt-E or PRKO-S + PRKO-E). These results clearly demonstrate that the inhibitory effect of P on uterine epithelial DNA synthesis is mediated by stromal PR. Epithelial PR is neither necessary nor sufficient for P inhibition of E2-induced epithelial DNA synthesis.

摘要

通过分析用野生型（wt）和孕激素受体基因敲除（PRKO）小鼠的子宫基质（S）和上皮（E）制备的四种类型的组织重组体，研究了上皮和基质孕激素（P）受体（PR）在P调节子宫上皮DNA合成中的作用：wt-S + wt-E、PRKO-S + PRKO-E、wt-S + PRKO-E和PRKO-S + wt-E。17-β雌二醇（E2）刺激了所有四种类型组织重组体中的DNA合成。另一方面，P仅在由野生型（PR阳性）基质制备的组织重组体（wt-S + wt-E或wt-S + PRKO-E）中抑制E2诱导的DNA合成，而在基因敲除（PR阴性）基质（PRKO-S + wt-E或PRKO-S + PRKO-E）中则无此作用。这些结果清楚地表明，P对子宫上皮DNA合成的抑制作用是由基质PR介导的。上皮PR对于P抑制E2诱导的上皮DNA合成既非必需也不充分。