Trouvé S, Coustau C
Centre de Biologie et d'Ecologie Tropicale et Méditerranéenne, Laboratoire de Biologie Animale, UMR 5555 du CNRS, Université de Perpignan, France.
J Parasitol. 1998 Oct;84(5):1062-5.
Because of the potentially important role of parasite excretory-secretory (ES) products in regulating intermate relationships and host-parasite interactions, we investigated intraspecific variability of adult ES products from a trematode species. Adults from 3 geographical isolates of Echinostoma caproni were collected and maintained under in vitro conditions. ES products were collected at 4, 8, and 22 hr of in vitro maintenance. In order to test for interspecific variability, ES products from a different echinostome species (Echinostoma sp.) were collected in a similar way. Major ES polypeptides were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis separation and silver staining. The polypeptide patterns of total ES products appeared very stable in time for each isolate. ES products from Echinostoma sp. showed a distinct polypeptide profile with none of the major bands being shared with the E. caproni isolates. Although polypeptide patterns from the 3 E. caproni isolates shared most major bands, isolate-specific bands could be observed. Two isolates exhibited a band at 85 and 119 kDa, respectively, whereas the third isolate was characterized by the absence of both bands. These results, together with the previously reported role of ES products in molecular signaling raise the question of the importance of intraspecific ES product differences in evolutionary processes such as assortative mating or local host adaptation.
由于寄生虫排泄-分泌（ES）产物在调节种内关系和宿主-寄生虫相互作用中可能发挥重要作用，我们研究了一种吸虫成虫ES产物的种内变异性。采集了来自卡氏棘口吸虫3个地理隔离种群的成虫，并在体外条件下进行饲养。在体外饲养4小时、8小时和22小时时收集ES产物。为了检测种间变异性，以类似方式收集了来自另一种棘口吸虫（棘口吸虫属）的ES产物。主要的ES多肽通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离和银染进行表征。每个分离种群的总ES产物的多肽图谱在时间上显得非常稳定。棘口吸虫属的ES产物显示出独特的多肽谱，没有一条主要条带与卡氏棘口吸虫分离种群共享。虽然来自卡氏棘口吸虫3个分离种群的多肽图谱共享了大多数主要条带，但可以观察到分离种群特异性条带。两个分离种群分别在85 kDa和119 kDa处出现一条条带，而第三个分离种群的特征是这两条带均不存在。这些结果，连同先前报道的ES产物在分子信号传导中的作用，引发了关于种内ES产物差异在诸如选型交配或局部宿主适应等进化过程中的重要性的问题。
