Institute of Immunology & Infection Research, School of Biological Sciences, University of Edinburgh, Ashworth Laboratories, King's Buildings, West Mains Rd, Edinburgh, EH9 3JT, UK.
Parasitology. 2011 Oct;138(12):1607-19. doi: 10.1017/S0031182011000412. Epub 2011 Jun 10.
With the current paucity of vaccine targets for parasitic diseases, particularly those in childhood, the aim of this study was to compare protein expression and immune cross-reactivity between the trematodes Schistosoma haematobium, S. bovis and Echinostoma caproni in the hope of identifying novel intervention targets. Native adult parasite proteins were separated by 2-dimensional gel electrophoresis and identified through electrospray ionisation tandem mass spectrometry to produce a reference gel. Proteins from differential gel electrophoresis analyses of the three parasite proteomes were compared and screened against sera from hamsters infected with S. haematobium and E. caproni following 2-dimensional Western blotting. Differential protein expression between the three species was observed with circa 5% of proteins from S. haematobium showing expression up-regulation compared to the other two species. There was 91% similarity between the proteomes of the two Schistosoma species and 81% and 78·6% similarity between S. haematobium and S. bovis versus E. caproni, respectively. Although there were some common cross-species antigens, species-species targets were revealed which, despite evolutionary homology, could be due to phenotypic plasticity arising from different host-parasite relationships. Nevertheless, this approach helps to identify novel intervention targets which could be used as broad-spectrum candidates for future use in human and veterinary vaccines.
由于寄生虫病（尤其是儿童寄生虫病）的疫苗靶点稀缺，本研究旨在比较曼氏血吸虫、牛血吸虫和埃及棘口吸虫的成虫蛋白表达和免疫交叉反应性，以期鉴定新的干预靶点。通过二维凝胶电泳分离天然成虫蛋白，并通过电喷雾离子化串联质谱鉴定，产生参考凝胶。通过对这三种寄生虫蛋白质组的差异凝胶电泳分析进行比较，并通过二维 Western blot 分析针对感染曼氏血吸虫和埃及棘口吸虫的仓鼠血清进行筛选。与其他两种物种相比，约 5%的曼氏血吸虫蛋白表达上调，观察到三种物种之间的差异蛋白表达。两种曼氏血吸虫物种的蛋白质组之间有 91%的相似性，而曼氏血吸虫和牛血吸虫与埃及棘口吸虫之间的相似性分别为 81%和 78.6%。虽然存在一些共同的交叉物种抗原，但也揭示了物种间的靶点，尽管存在进化同源性，但这可能是由于来自不同宿主-寄生虫关系的表型可塑性所致。尽管如此，这种方法有助于鉴定新的干预靶点，这些靶点可以作为广谱候选物，用于未来的人类和兽医疫苗。
