Williams S B, Sano M, Smith N, Horne M, Yarchoan R, Wyvill K, Zeichner S, Taylor P, Knudson T, Gralnick H R
Warren G. Magnuson Clinical Center and the HIV and Aids Malignancy Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
J Lab Clin Med. 1998 Oct;132(4):303-7. doi: 10.1016/s0022-2143(98)90043-9.
Glycocalicin (GC) is the carbohydrate-rich portion of platelet membrane glycoprotein Ib(alpha) that can be cleaved from circulating platelets by proteases. The plasma GC level is an indicator of platelet turnover. Using an ELISA for GC, we assayed the plasma of 20 normal children (age 6 to 13 years), 50 HIV+ children (ages 4 to 18 years), 32 normal adults (ages 21 to 53 years), and 50 HIV+ adults (ages 24 to 66 years). The results were adjusted for individual platelet counts to give GC indexes (GCI). The normal children and the normal adults had significantly different GCI distributions (P = .002). In both normal and HIV+ individuals the GCI decreased with increasing platelet count (-.73 < r < -.34). Twenty-eight percent of the HIV+ children and 28% of the HIV+ adults had elevated GCI values. The majority of these elevated values occurred in patients with platelet counts >100,000/microL. Neither the GCI nor the platelet count was correlated with viral load. The platelet count, however, was weakly correlated with the CD4 count in both children (r = .31) and adults (r = .30) infected with HIV. Also, the CD4 count was weakly and inversely correlated with GCI in HIV+ adults (r = -.34) and in children (r = -.24). We conclude that increased GCI and, by implication, increased platelet turnover is a relatively common feature of advanced HIV disease. Furthermore, GCI may be elevated in HIV+ patients even with a platelet count >100,000/microL, suggesting increased platelet turnover before thrombocytopenia develops.
糖萼素（GC）是血小板膜糖蛋白Ib（α）富含碳水化合物的部分，可被蛋白酶从循环血小板中切割下来。血浆GC水平是血小板更新的一个指标。我们使用一种针对GC的酶联免疫吸附测定法（ELISA），检测了20名正常儿童（6至13岁）、50名HIV阳性儿童（4至18岁）、32名正常成年人（21至53岁）和50名HIV阳性成年人（24至66岁）的血浆。结果根据个体血小板计数进行了调整，以得出GC指数（GCI）。正常儿童和正常成年人的GCI分布有显著差异（P = 0.002）。在正常人和HIV阳性个体中，GCI均随血小板计数增加而降低（-0.73 < r < -0.34）。28%的HIV阳性儿童和28%的HIV阳性成年人GCI值升高。这些升高的值大多出现在血小板计数>100,000/微升的患者中。GCI和血小板计数均与病毒载量无关。然而，在感染HIV的儿童（r = 0.31）和成年人（r = 0.30）中，血小板计数与CD4计数呈弱相关。此外，在HIV阳性成年人（r = -0.34）和儿童（r = -0.24）中，CD4计数与GCI呈弱的负相关。我们得出结论，GCI升高以及由此暗示的血小板更新增加是晚期HIV疾病的一个相对常见特征。此外，即使血小板计数>100,000/微升，HIV阳性患者的GCI也可能升高，这表明在血小板减少症发生之前血小板更新就已增加。
