Yoon H S, Golden J W
Department of Biology, Texas A&M University, College Station, TX 77843-3258, USA.
Science. 1998 Oct 30;282(5390):935-8. doi: 10.1126/science.282.5390.935.
Many filamentous cyanobacteria grow as multicellular organisms that show a developmental pattern of single nitrogen-fixing heterocysts separated by approximately 10 vegetative cells. Overexpression of a 54-base-pair gene, patS, blocked heterocyst differentiation in Anabaena sp. strain PCC 7120. A patS null mutant showed an increased frequency of heterocysts and an abnormal pattern. Expression of a patS-gfp reporter was localized in developing proheterocysts. The addition of a synthetic peptide corresponding to the last five amino acids of PatS inhibited heterocyst development. PatS appears to control heterocyst pattern formation through intercellular signaling mechanisms.
许多丝状蓝细菌以多细胞生物的形式生长，呈现出由大约10个营养细胞分隔单个固氮异形胞的发育模式。一个54个碱基对的基因patS的过表达阻断了鱼腥藻属PCC 7120菌株中的异形胞分化。patS缺失突变体显示异形胞频率增加且模式异常。patS-gfp报告基因的表达定位于正在发育的前异形胞中。添加与PatS最后五个氨基酸对应的合成肽可抑制异形胞发育。PatS似乎通过细胞间信号传导机制控制异形胞模式的形成。
