Pileri P, Uematsu Y, Campagnoli S, Galli G, Falugi F, Petracca R, Weiner A J, Houghton M, Rosa D, Grandi G, Abrignani S
IRIS, Chiron, Siena 53100, Italy.
Science. 1998 Oct 30;282(5390):938-41. doi: 10.1126/science.282.5390.938.
Chronic hepatitis C virus (HCV) infection occurs in about 3 percent of the world's population and is a major cause of liver disease. HCV infection is also associated with cryoglobulinemia, a B lymphocyte proliferative disorder. Virus tropism is controversial, and the mechanisms of cell entry remain unknown. The HCV envelope protein E2 binds human CD81, a tetraspanin expressed on various cell types including hepatocytes and B lymphocytes. Binding of E2 was mapped to the major extracellular loop of CD81. Recombinant molecules containing this loop bound HCV and antibodies that neutralize HCV infection in vivo inhibited virus binding to CD81 in vitro.
慢性丙型肝炎病毒（HCV）感染发生在全球约3%的人口中，是肝脏疾病的主要病因。HCV感染还与冷球蛋白血症相关，冷球蛋白血症是一种B淋巴细胞增殖性疾病。病毒嗜性存在争议，细胞进入机制仍不清楚。HCV包膜蛋白E2与人CD81结合，CD81是一种在包括肝细胞和B淋巴细胞在内的多种细胞类型上表达的四跨膜蛋白。E2的结合定位在CD81的主要细胞外环。含有该环的重组分子能结合HCV，并且在体内中和HCV感染的抗体在体外可抑制病毒与CD81的结合。
