Elewaut D, Van Damme N, De Keyser F, Baeten D, De Paepe P, Van Vlierberghe H, Mielants H, Cuvelier C, Verbruggen G, Veys E M, De Vos M
Department of Rheumatology, University Hospital Ghent, Belgium.
Acta Gastroenterol Belg. 1998 Jul-Sep;61(3):288-94.
To compare the expression of adhesion molecules on intestinal intra-epithelial (IEL) and lamina propria T cells (LPL) from ileum and colon, in patients with Crohn's disease (CD) versus healthy controls, with special reference for the alpha E beta 7 integrin.
IEL and LPL were obtained from 18 CD patients and 20 controls by enzymatic extraction, and subsequently characterized by flow cytometry for CD3, CD4, CD8, CD25, LFA-1 alpha (CD11a), CD44, alpha 4 and alpha E beta 7 integrin.
In LPL of controls, a decreased CD4/CD8 ratio was noted in ileum compared to colon. This regional difference was accompanied by a higher expression of alpha E beta 7 integrin in ileum versus colon. In LPL from left hemicolon of CD patients, a decreased CD4/CD8 ratio was noted versus controls. alpha E beta 7 expression on T cells of LPL did not discriminate CD from controls. However, an overexpression of this beta 7 integrin member was observed on CD25+ T cell subsets from lamina propria of left hemicolon, in CD versus controls. Moreover, in IEL, profound alterations in alpha E beta 7 integrin were observed in CD, compared to controls. A decreased expression of alpha E beta 7 was noted in IEL of ileum of CD patients. This was also apparent in non-inflamed mucosa.
The observed changes of alpha E beta 7 integrin expression in CD patients versus controls are of pathogenic relevance, especially the decreased expression of alpha E beta 7 in IEL of non-inflamed CD mucosa. This may be one of the earliest events in the pathogenesis of this disease.
比较克罗恩病（CD）患者与健康对照者回肠和结肠的肠上皮内淋巴细胞（IEL）及固有层T细胞（LPL）上黏附分子的表达情况，特别关注αEβ7整合素。
通过酶提取法从18例CD患者和20例对照者获取IEL和LPL，随后用流式细胞术对CD3、CD4、CD8、CD25、淋巴细胞功能相关抗原-1α（CD11a）、CD44、α4和αEβ7整合素进行鉴定。
在对照者的LPL中，回肠的CD4/CD8比值低于结肠。这种区域差异伴随着回肠中αEβ7整合素表达高于结肠。在CD患者左半结肠的LPL中，与对照者相比CD4/CD8比值降低。LPL中T细胞上的αEβ7表达不能区分CD患者与对照者。然而，与对照者相比，在CD患者左半结肠固有层的CD25 + T细胞亚群上观察到该β7整合素成员的过表达。此外，在IEL中，与对照者相比，CD患者的αEβ7整合素发生了显著改变。在CD患者回肠的IEL中αEβ7表达降低。这在非炎症黏膜中也很明显。
观察到的CD患者与对照者之间αEβ7整合素表达的变化具有致病相关性，尤其是非炎症性CD黏膜IEL中αEβ7表达降低。这可能是该疾病发病机制中最早的事件之一。
