Sjölander A, Baldwin T M, Curtis J M, Bengtsson K L, Handman E
CSL Limited, Parkville, Victoria, Australia.
Vaccine. 1998 Dec;16(20):2077-84. doi: 10.1016/s0264-410x(98)00075-9.
Vaccination with the native Parasite Surface Antigen 2 of Leishmania major with Corynebacterium parvum as adjuvant protects mice from leishmaniasis through a Th1 mediated response. Here we show that vaccination with a recombinant form of this protein, purified from Escherichia coli and administered in iscoms or with C. parvum as adjuvant, does not induce protective immunity despite the induction of Th1 responses. The results suggest that protective immunity depends on the ability of the vaccinating antigen to induce Th1-like T cells with ability to be recalled by infection. Therefore, the conformation of antigens may play a more major role for the induction of T cell mediated immunity than originally considered.
用利什曼原虫主要表面抗原2（Leishmania major Parasite Surface Antigen 2）与短小棒状杆菌（Corynebacterium parvum）作为佐剂进行疫苗接种，可通过Th1介导的反应保护小鼠免受利什曼病感染。在此我们表明，用从大肠杆菌中纯化并以免疫刺激复合物（iscoms）形式或与短小棒状杆菌作为佐剂给药的该蛋白重组形式进行疫苗接种，尽管诱导了Th1反应，但并未诱导出保护性免疫。结果表明，保护性免疫取决于接种抗原诱导具有被感染激活能力的Th1样T细胞的能力。因此，抗原的构象在诱导T细胞介导的免疫中可能比最初认为的发挥更重要的作用。
