Elsgaard L
Department of Soil Science and Crop Physiology, Danish Institute of Agricultural Sciences, Research Center Foulum, DK-8830 Tjele, Denmark.
Appl Environ Microbiol. 1998 Nov;64(11):4168-73. doi: 10.1128/AEM.64.11.4168-4173.1998.
A biofilter which eliminated ethylene (C2H4) from the high parts-per-million range to levels near the limit for plant hormonal activity (0.01 to 0.1 ppm) was developed. Isolated ethylene-oxidizing bacteria were immobilized on peat-soil in a biofilter (687 cm3) and subjected to an atmospheric gas flow (73.3 ml min-1) with 2 or 117 ppm of C2H4. Ethylene was eliminated to a minimum level of 0.017 ppm after operation with 2.05 ppm of C2H4 for 16 days. Also, the inlet C2H4 concentration of 117 ppm was reduced to <0.04 ppm. During operation with 2 and 117 ppm of C2H4, an increase in the C2H4 removal rate was observed, which was attributed to proliferation of the immobilized bacteria, notably in the first 0- to 5-cm segment of the biofilter. The maximal C2H4 elimination capacity of the biofilter was 21 g of C2H4 m-3 day-1 during operation with 117 ppm of C2H4 in the inlet gas. However, for the first 0- to 5-cm segment of the biofilter, an elimination capacity of 146 g of C2H4 m-3 day-1 was calculated. Transition of the biofilter temperature from 21 to 10 degreesC caused a 1.6-fold reduction in the C2H4 removal rate, which was reversed during operation for 18 days. Batch experiments with inoculated peat-soil demonstrated that C2H4 removal still occurred after storage at 2, 8, and 20 degreesC for 2, 3, and 4 weeks. However, the C2H4 removal rate decreased with increasing storage time and was reduced by ca. 50% after storage for 2 weeks at all three temperatures. The biofilter could be a suitable tool for C2H4 removal in, e.g., horticultural storage facilities, since it (i) removed C2H4 to 0.017 ppm, (ii) had a good operational stability, and (iii) operated efficiently at 10 degreesC.
研发出了一种生物滤器，它能将百万分率含量处于高位的乙烯（C₂H₄）去除至接近植物激素活性极限水平（0.01至0.1 ppm）。将分离出的乙烯氧化细菌固定在一个生物滤器（687立方厘米）的泥炭土上，并使其接触含有2 ppm或117 ppm C₂H₄的大气气流（73.3毫升/分钟）。在以2.05 ppm的C₂H₄运行16天后，乙烯被去除至最低水平0.017 ppm。此外，117 ppm的入口C₂H₄浓度降至<0.04 ppm。在以2 ppm和117 ppm的C₂H₄运行期间，观察到C₂H₄去除率有所增加，这归因于固定化细菌的增殖，尤其是在生物滤器最初0至5厘米的部分。在入口气体中C₂H₄含量为117 ppm的运行期间，该生物滤器的最大C₂H₄去除能力为21克C₂H₄/立方米·天。然而，对于生物滤器最初0至5厘米的部分，计算得出的去除能力为146克C₂H₄/立方米·天。生物滤器温度从21℃降至10℃导致C₂H₄去除率降低了1.6倍，在运行18天后这种情况得到逆转。对接种泥炭土进行的批次实验表明，在2℃、8℃和20℃下分别储存2周、3周和4周后，仍然能够去除C₂H₄。然而，C₂H₄去除率随着储存时间的增加而降低，在所有这三个温度下储存2周后，去除率均降低了约50%。该生物滤器可能是例如园艺储存设施中去除C₂H₄的合适工具，因为它（i）能将C₂H₄去除至0.017 ppm，（ii）具有良好的运行稳定性，并且（iii）在10℃下能高效运行。
