Kobzev Y, Domracheva E, Zakharova A, Khoroshko N, Turkina A, Dewald G
Karyology Laboratory, National Research Center for Hematology, Moscow, Russia.
Cancer Genet Cytogenet. 1998 Oct 15;106(2):128-34. doi: 10.1016/s0165-4608(98)00052-1.
We used two-color fluorescence in situ hybridization (FISH) to detect BCR/ABL fusion in interphase nuclei in bone marrow of 17 patients with chronic myeloid leukemia (CML) before and in the course of interferon therapy. The results of FISH were compared with the data of conventional cytogenetic investigation (G- or Q-banding) of the same specimens. Changes in percentage of Ph-positive nuclei correlated with variations in percentage of Ph-positive metaphases. An overall difference in the classification of patients by conventional cytogenetics and FISH based on the percentage of Ph-positive cells was not observed. This FISH method is reproducible, relatively easy to perform, and reliable for monitoring patients with CML.
我们采用双色荧光原位杂交（FISH）技术，对17例慢性髓性白血病（CML）患者骨髓间期核中的BCR/ABL融合情况进行治疗前及干扰素治疗过程中的检测。将FISH结果与同一标本的常规细胞遗传学检查（G显带或Q显带）数据进行比较。Ph阳性核百分比的变化与Ph阳性中期细胞百分比的变化相关。基于Ph阳性细胞百分比，常规细胞遗传学和FISH对患者的分类总体上未观察到差异。这种FISH方法具有可重复性，操作相对简便，对CML患者的监测可靠。
