Ntirampemba G, Langlois B E, Archbold D D, Hamilton-Kemp T R, Barth M M
Department of Animal Sciences, University of Kentucky, Lexington 40546-0215, USA.
J Food Prot. 1998 Oct;61(10):1352-7. doi: 10.4315/0362-028x-61.10.1352.
Aerobic, microaerophilic, coliform, and mold populations of Botrytis cinerea-inoculated strawberry fruit not exposed (control) or exposed to low and high quantities of four volatile compounds during storage at 2 degrees C were determined after storage for 7 days and after removal of the volatile and transfer to 22 degrees C for 3 days. Fruit harvested at the ripe stage were inoculated with 10(6) conidia B. cinerea per ml and were placed in plastic containers containing no volatile compound (control) or two quantities of (E)-2-hexenal (10 or 100 microliters), (E)-2-hexenal diethyl acetal (30 or 300 microliters), benzaldehyde (30 or 300 microliters), or methyl benzoate (12 or 60 microliters). The fruit containers were overwrapped with a low-density polyethylene film, sealed, stored at 2 degrees C for 7 days, and then transferred to 22 degrees C for 3 days. Aerobic, microaerophilic, and coliform populations of fruit exposed to volatile compounds tended to be lower than the controls after storage at 2 degrees C for 7 days and, depending on the volatile compound, similar, lower, or higher than the controls after transfer and storage at 22 degrees C. However, due to variability in initial aerobic, microaerophilic, and coliform populations of the fruit used in the different trials (P < 0.05), none of the differences between control and treatment and between treatments within a sample time were significant (P > 0.05). Strawberry fruit exposed to 100 microliters of (E)-2-hexenal was the only treatment that did not show a significant increase in mold populations after transfer and storage at 22 degrees C for 3 days. Additional studies are needed to determine if (E)-2-hexenal can be used in combination with other postharvest storage conditions, such as low temperature and controlled/modified atmosphere, to delay mold spoilage and extend the shelf life of the strawberry.
在2℃储存7天后以及去除挥发性化合物并转移至22℃再储存3天后，测定了接种灰霉病菌的草莓果实的需氧菌、微需氧菌、大肠菌群和霉菌数量，这些草莓果实未暴露（对照）或在2℃储存期间暴露于低量和高量的四种挥发性化合物中。在成熟阶段采收的果实接种每毫升含10⁶个灰霉病菌分生孢子，并置于不含有挥发性化合物（对照）或含有两种量的（E）-2-己烯醛（10或100微升）、（E）-2-己烯醛二乙缩醛（30或300微升）、苯甲醛（30或300微升）或苯甲酸甲酯（12或60微升）的塑料容器中。果实容器用低密度聚乙烯薄膜包裹，密封，在2℃储存7天，然后转移至22℃储存3天。在2℃储存7天后，暴露于挥发性化合物的果实的需氧菌、微需氧菌和大肠菌群数量往往低于对照，并且根据挥发性化合物的不同，在转移至22℃并储存后，与对照相似、低于或高于对照。然而，由于不同试验中使用的果实初始需氧菌、微需氧菌和大肠菌群数量存在差异（P<0.05），对照与处理之间以及样品时间内各处理之间的差异均不显著（P>0.05）。暴露于100微升（E）-2-己烯醛的草莓果实是唯一在转移至22℃并储存3天后霉菌数量没有显著增加的处理。需要进一步研究以确定（E）-2-己烯醛是否可以与其他采后储存条件（如低温和控制/调节气氛）结合使用，以延迟霉菌腐败并延长草莓的货架期。
