Asturias J A, Arilla M C, Gómez-Bayón N, Aguirre M, Martínez A, Palacios R, Martínez J
R&D Department, IFIDESA-ARISTEGUI, Alameda Urquijo, Bilbao, Spain.
Mol Immunol. 1998 Jun;35(8):469-78. doi: 10.1016/s0161-5890(98)00036-4.
Sunflower (Helianthus annuus) sensitization is not always related with occupational allergy. We have isolated the allergen profilin (Hel a 2) from this Compositae plant, cloned and sequenced five cDNAs encoding for full-length or partial Hel a 2. Natural sunflower profilin reacted with specific IgE in the 121 sera tested, at a frequency of 30.5%. Expression of the cDNA encoding Hel a 2 in Escherichia coli and a simple purification procedure by poly-L-proline chromatography allowed immunological characterization of the recombinant allergen. Binding of monoclonal antibodies against sunflower profilin revealed that some epitopes responsible for antigen-specific IgG production were not present in the recombinant allergen. High cross-reactivity has been found between recombinant Hel a 2 and profilins from other Compositae plants and also from botanically distant plants.
向日葵（Helianthus annuus）致敏并不总是与职业性过敏相关。我们从这种菊科植物中分离出了变应原肌动蛋白结合蛋白（Hel a 2），克隆并测序了五个编码全长或部分Hel a 2的cDNA。在所检测的121份血清中，天然向日葵肌动蛋白结合蛋白与特异性IgE发生反应，频率为30.5%。编码Hel a 2的cDNA在大肠杆菌中的表达以及通过聚-L-脯氨酸色谱法进行的简单纯化程序，使得重组变应原的免疫学特性得以确定。针对向日葵肌动蛋白结合蛋白的单克隆抗体的结合表明，重组变应原中不存在一些负责产生抗原特异性IgG的表位。已发现重组Hel a 2与其他菊科植物以及植物分类学上亲缘关系较远的植物中的肌动蛋白结合蛋白之间具有高度交叉反应性。
