通过染色体涂染分析人类染色体的DNA含量与其在辐射诱导的结构畸变中的作用之间的关系。
Relationship between the DNA content of human chromosomes and their involvement in radiation-induced structural aberrations, analysed by painting.
作者信息
Cigarrán S, Barrios L, Barquinero J F, Caballín M R, Ribas M, Egozcue J
机构信息
Dpt. Biologia Animal, Biologia Vegetal i Ecologia, Facultat de Ciències, Universitat Autònoma de Barcelona, Bellaterra, Spain.
出版信息
Int J Radiat Biol. 1998 Oct;74(4):449-55. doi: 10.1080/095530098141311.
PURPOSE
To study the relationship between the DNA content of human chromosomes and their involvement in radiation-induced structural chromosome aberrations.
MATERIAL AND METHODS
Human lymphocytes were cultured after exposure to 5 Gy of X-rays. FISH-painting was performed for all human chromosomes.
RESULTS
The results indicate that with the exception of chromosome 20, there was a good fit between the DNA content and the number of exchange-type aberrations and the number of breaks. However, there was a significant tendency for short chromosomes to be more affected than expected and for long chromosomes to be less affected than expected. A better fit was observed when, instead of DNA content, the surface area of the chromosome territories obtained from a spherical model was taken into consideration.
CONCLUSIONS
The tendency for short chromosomes to be more involved and long chromosomes to be less involved in exchange-type aberrations could be related not only to their DNA content but also to their interphase territory surface area.
目的
研究人类染色体的DNA含量与其在辐射诱导的染色体结构畸变中的参与情况之间的关系。
材料与方法
人类淋巴细胞在接受5 Gy的X射线照射后进行培养。对所有人类染色体进行荧光原位杂交（FISH）染色体涂染。
结果
结果表明，除了20号染色体外，DNA含量与交换型畸变的数量以及断裂的数量之间存在良好的拟合关系。然而，存在一个显著的趋势，即短染色体比预期更易受影响，而长染色体比预期受影响更小。当考虑从球形模型获得的染色体区域的表面积而非DNA含量时，观察到了更好的拟合。
结论
短染色体在交换型畸变中更易参与、长染色体参与较少的趋势不仅可能与其DNA含量有关，还可能与其间期区域表面积有关。
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