Blixt A, Mahlapuu M, Bjursell C, Darnfors C, Johannesson T, Enerbäck S, Carlsson P
Department of Molecular Biology, Göteborg University, Göteborg, Medicinaregatan 9C, S-413 90, Sweden.
Genomics. 1998 Nov 1;53(3):387-90. doi: 10.1006/geno.1998.5451.
The gene for the human transcription factor forkhead related activator 2 (FREAC-2; HGMW-approved symbol FKHL6) has been characterized and found to consist of two exons separated by an intron of 3.6 kb. The first exon encodes the forkhead DNA-binding domain and one of the transcriptional activation domains, AD2. The second exon contains the coding sequence corresponding to the C-terminal activation domain AD1. The full-length FREAC-2 protein is predicted to be 444 amino acids, which adds 39 amino acids to the previously published partial cDNA sequence. A 2-kb CG island is centered around the 5' end of the FREAC-2 gene. Fluorescence in situ hybridization was used to localize the human FREAC-2 gene to chromosomal position 6p24-p25, and the localization was further refined by radiation hybrid mapping to 6p25.3.
人类转录因子叉头相关激活因子2（FREAC-2；HGMW批准符号为FKHL6）的基因已被鉴定，发现它由两个外显子组成，中间隔着一个3.6kb的内含子。第一个外显子编码叉头DNA结合结构域和一个转录激活结构域AD2。第二个外显子包含与C端激活结构域AD1相对应的编码序列。全长FREAC-2蛋白预计有444个氨基酸，比先前发表的部分cDNA序列多39个氨基酸。一个2kb的CG岛以FREAC-2基因的5'端为中心。荧光原位杂交用于将人类FREAC-2基因定位到染色体位置6p24-p25，通过辐射杂种图谱进一步精确定位到6p25.3。
