Stangl G I, Kirchgessner M
Institut für Ernährungsphysiologie Technische Universität München-Weihenstephan, Freising.
Z Ernahrungswiss. 1998 Sep;37(3):260-8. doi: 10.1007/s003940050025.
Severe iron deficiency results in complex systemic disorders e.g., including metabolism of energy and minerals. To investigate whether also moderate iron depletion may alter the activities of citric cycle enzymes and the cytochrome oxidase, the trace element status, and serum enzymes indicative of cell damage, this experiment was carried out with rats supplied with sub-optimal iron (9, 13 and 18 mg iron per kg diet) over a total of 5 weeks. The study included 3 pair-fed groups and an ad libitum group, fed with 50 mg iron/kg diet. All iron-restricted rats were classified as iron-deficient on the basis of reduced iron concentrations in body and iron-depending blood parameters. Body weight gain and catalase activity in kidney were lowered in rats receiving the lowest dietary iron level, exclusively. Rats fed 9 and 13 mg iron per kg diet had nearly 6- and 3-fold, respectively higher platelet counts in blood than their corresponding pair-fed controls. The activities of transaminases ASAT and ALAT, alkaline phosphatase, glutamate dehydrogenase and lactate dehydrogenase in serum which are indicative of cell damage were also markedly influenced by moderate dietary iron restriction, in which the enzyme levels in serum increased with intensifying iron depletion. Although, moderate iron restriction to young male rats was associated with marked alterations in iron status and serum enzymes, the activities of tricarboxylic acid cycle enzymes including malic dehydrogenase, fumarase, and isocitric dehydrogenase as well as cytochrome oxidase in liver remained largely unaffected. Only hepatic aconitase showed a somewhat reduction with iron depletion. Moreover, iron restriction was also accompanied with an accumulation of copper in liver which was significant for rats fed 9 and 13 mg iron per kg diet, whereas zinc status remained completely unaffected by moderate iron deficiency. It can be concluded, that a short-term moderate iron deficiency with ranging hemoglobin concentrations from 66 and 121 g/L, was accompanied with altered platelet counts, serum enzyme activities indicative of cell damage, and hepatic copper concentrations, but the activities of the tricarboxylic acid cycle enzymes and cytochrome oxidase in liver remained largely unaffected.
严重缺铁会导致复杂的全身性疾病，例如包括能量和矿物质代谢。为了研究中度铁缺乏是否也会改变柠檬酸循环酶和细胞色素氧化酶的活性、微量元素状态以及指示细胞损伤的血清酶，本实验对大鼠进行了为期5周的次优铁供应（每千克饮食含9、13和18毫克铁）。该研究包括3个配对喂养组和1个自由采食组，自由采食组喂食每千克饮食含50毫克铁的食物。所有铁限制大鼠根据体内铁浓度降低和依赖铁的血液参数被归类为缺铁。仅接受最低饮食铁水平的大鼠体重增加和肾脏过氧化氢酶活性降低。每千克饮食喂食9毫克和13毫克铁的大鼠血液中的血小板计数分别比相应的配对喂养对照高近6倍和3倍。指示细胞损伤的血清中转氨酶ASAT和ALAT、碱性磷酸酶、谷氨酸脱氢酶和乳酸脱氢酶的活性也受到中度饮食铁限制的显著影响，其中血清中的酶水平随着铁缺乏加剧而增加。尽管对年轻雄性大鼠进行中度铁限制与铁状态和血清酶的显著改变有关，但肝脏中包括苹果酸脱氢酶、延胡索酸酶和异柠檬酸脱氢酶在内的三羧酸循环酶以及细胞色素氧化酶的活性在很大程度上未受影响。只有肝脏乌头酸酶随着铁缺乏而略有降低。此外铁限制还伴随着肝脏中铜的积累，这对于每千克饮食喂食9毫克和13毫克铁的大鼠来说是显著的，而锌状态在中度缺铁情况下完全未受影响。可以得出结论，短期中度缺铁（血红蛋白浓度范围为66至121克/升）伴随着血小板计数改变、指示细胞损伤的血清酶活性以及肝脏铜浓度的变化，但肝脏中三羧酸循环酶和细胞色素氧化酶的活性在很大程度上未受影响。
