猪蛋白酶体亚基A4（PSMA4）基因：部分cDNA的分离、连锁分析和物理图谱绘制

The porcine proteasome subunit A4 (PSMA4) gene: isolation of a partial cDNA, linkage and physical mapping.

作者信息

Davoli R, Fontanesi L, Russo V, Cepica S, Musilová P, Stratil A, Rubes J

机构信息

DIPROVAL, Sezione di Allevamenti Zootecnici, Faculty of Agriculture, University of Bologna, Reggio Emilia, Italy.

出版信息

Anim Genet. 1998 Oct;29(5):385-8. doi: 10.1046/j.1365-2052.1998.295354.x.

DOI:10.1046/j.1365-2052.1998.295354.x

PMID:9800328

Abstract

A partial cDNA clone encoding the porcine proteasome subunit A4 (PSMA4 or proteasome subunit C9) has been isolated from a porcine muscle cDNA library and sequenced. A biallelic TaqI RFLP was identified in Large White, Landrace and Duroc breeds. Moreover, the 3'-untranslated region of the gene showed a triallelic SSCP. By linkage analysis the PSMA4 locus was assigned to pig chromosome 7 and by radioactive in situ hybridization this locus was mapped to the region 7q13-q14.

摘要

已从猪肌肉cDNA文库中分离并测序了一个编码猪蛋白酶体亚基A4（PSMA4或蛋白酶体亚基C9）的部分cDNA克隆。在大白猪、长白猪和杜洛克猪品种中鉴定出一个双等位基因TaqI限制性片段长度多态性（RFLP）。此外，该基因的3'-非翻译区显示出三等位基因单链构象多态性（SSCP）。通过连锁分析，将PSMA4基因座定位到猪的7号染色体上，通过放射性原位杂交，该基因座被定位到7q13-q14区域。