Osawa M, Yukawa N, Saito T, Huang X L, Kusakabe T, Takeichi S
Department of Forensic Medicine, Tokai University School of Medicine, Kanagawa, Japan.
Forensic Sci Int. 1998 Aug 31;96(1):39-45. doi: 10.1016/s0379-0738(98)00106-6.
Gc globulin binds actin at an equimolar ratio with a high affinity. It has been revealed that complexes of Gc with actin are present in the plasma of human cadavers. In the present study, an immunoassay which we developed using a specific anti-Gc monoclonal antibody was applied to evaluate the complexed Gc in cadaver plasma. The percentage of Gc in the complexed form was found to be 38.3 +/- 19.8% (n = 45), and a significantly higher percentage was observed in the plasma of cadavers from burn death. However, the plot of the complex percentage against the time since death showed a relatively low correlation, with a coefficient (r) of 0.51. This was supposed to be due to different environmental conditions after death. Since the complex formation represents the release of cytoplasmic actin into plasma from collapsed cells, the phenomenon should be noted in forensic postmortem chemistry.
Gc球蛋白以等摩尔比与肌动蛋白高亲和力结合。已发现Gc与肌动蛋白的复合物存在于人体尸体的血浆中。在本研究中，我们使用特异性抗Gc单克隆抗体开发的免疫测定法用于评估尸体血浆中复合形式的Gc。发现复合形式的Gc百分比为38.3±19.8%（n = 45），在烧伤死亡尸体的血浆中观察到显著更高的百分比。然而，复合百分比与死亡时间的关系图显示相关性相对较低，系数（r）为0.51。这被认为是由于死后不同的环境条件所致。由于复合物的形成代表细胞质肌动蛋白从破裂细胞释放到血浆中，这种现象在法医死后化学中应予以注意。
