Nakabayashi T, Kumagai T, Sakagami S, Furihata K, Katuyama T
Central Clinical Laboratory, Shinshu University Hospital, Matsumoto.
Rinsho Byori. 1998 Sep;46(9):942-7.
Antinuclear antibodies (ANAs) are clinically important indicators of collagen diseases. As corresponding antigens for ANAs vary considerably, patients with collagen diseases usually demonstrate several ANAs coincidentally, making difficult to detect the full spectrum of ANAs in each patient's serum. To design an efficient system for measuring ANAs, an enzyme-linked immunosorbent assay (ELISA) which adsorbs eight kinds of recombinant or purified antigens in each well of a multiwell plate was used and results were compared to those obtained with conventional assays by the fluorescent antinuclear antibodies (FANA), and double immunodiffusion (DID) methods. The positivity rates of 106 sera from patients with collagen diseases and 286 sera from healthy subjects were 92.5% and 5.5%, respectively. Sixty-one of 65 positive sera (93.8%) in the corresponding ANAs positive sera by DID or other conventional assay methods were positive by ELISA. Anti-SSA/Ro antibody could be detected with higher sensitivity by this assay method than with the FANA and DID method, but the sensitivities for anti-Scl-70 antibody and anti-centromere antibody were lower. Application of this ELISA method for measuring ANAs along with the FANA test may be beneficial for diagnosis of collagen diseases.
抗核抗体（ANA）是胶原病的重要临床指标。由于ANA的相应抗原差异很大，胶原病患者通常会同时出现几种ANA，因此难以检测出每位患者血清中ANA的全貌。为设计一种高效的ANA检测系统，采用了一种酶联免疫吸附测定（ELISA）方法，该方法在多孔板的每个孔中吸附八种重组或纯化抗原，并将结果与通过荧光抗核抗体（FANA）和双向免疫扩散（DID）方法的传统检测结果进行比较。106例胶原病患者血清和286例健康受试者血清的阳性率分别为92.5%和5.5%。在DID或其他传统检测方法对应的ANA阳性血清中，65份阳性血清中有61份（93.8%）通过ELISA检测为阳性。与FANA和DID方法相比，该检测方法检测抗SSA/Ro抗体的灵敏度更高，但抗Scl - 70抗体和抗着丝点抗体的灵敏度较低。将这种ELISA方法与FANA检测一起应用于ANA检测可能有助于胶原病的诊断。
