Reindl A, Schöffl F
Universität Tübingen, Biologisches Institut, Lehrstuhl für Allgemeine Genetik, Germany.
FEBS Lett. 1998 Oct 9;436(3):318-22. doi: 10.1016/s0014-5793(98)01152-1.
The heat shock factor (HSF1) is the central regulator of the heat stress (hs) response and is required for stimulating the transcription of the hs genes and consequently the expression of heat shock proteins. To promote the polymerase II-dependent transcription of the hs genes, HSF has to communicate with the basal transcription machinery. Here, we report that the Arabidopsis thaliana HSF1 interacts directly with TBP, the general TATA box binding transcription factor, as shown by affinity chromatography and electrophoretic mobility shift analyses in vitro. An in vivo interaction between AtHSF1 and AtTBP1 was suggested by results employing the yeast two-hybrid system.
热休克因子（HSF1）是热应激（hs）反应的核心调节因子，对于刺激热休克基因的转录以及随后热休克蛋白的表达是必需的。为了促进热休克基因的聚合酶II依赖性转录，热休克因子必须与基础转录机制进行通讯。在此，我们报告拟南芥HSF1与TBP直接相互作用，TBP是一般的TATA盒结合转录因子，体外亲和层析和电泳迁移率变动分析表明了这一点。利用酵母双杂交系统的结果提示了AtHSF1和AtTBP1之间的体内相互作用。
