Knoetzel J, Bossmann B, Grimme L H
Institute of Cell Biology, Biochemistry and Biotechnology, University of Bremen, Germany.
FEBS Lett. 1998 Oct 9;436(3):339-42. doi: 10.1016/s0014-5793(98)01158-2.
The isolated subcomplex LHCI-730 of plant photosystem I (PSI) chlorophyll (Chl) alb binding antenna is a heterodimer of Lhca1 and Lhca4 and has a 77 K fluorescence emission peak at 730 nm (F730). Recently, three Chl spectral forms with 77 K fluorescence emission peaks at 720 nm, 730 nm and 742 nm were identified in native PSI. In an attempt to assign the two longest wavelength emission maxima to peripheral PSI antenna proteins, we performed 77 K fluorescence emission spectroscopy on intact leaves of chlorina and viridis mutants from barley which lack individual LHCI-730 proteins. This approach indicates that F732 is found only in Lhca1 and F742 only in Lhca4, when these proteins are associated with the PSI reaction centre.
植物光系统I（PSI）叶绿素（Chl）-alb结合天线的分离亚复合物LHCI-730是Lhca1和Lhca4的异二聚体，在730 nm处有一个77 K荧光发射峰（F730）。最近，在天然PSI中鉴定出了三种在720 nm、730 nm和742 nm处有77 K荧光发射峰的Chl光谱形式。为了将两个最长波长的发射最大值归因于外周PSI天线蛋白，我们对大麦的chlorina和viridis突变体的完整叶片进行了77 K荧光发射光谱分析，这些突变体缺乏单个LHCI-730蛋白。这种方法表明，当这些蛋白与PSI反应中心结合时，F732仅在Lhca1中出现，F742仅在Lhca4中出现。
