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Inhibition of the double-stranded RNA-dependent protein kinase PKR by mammalian ribosomes.

作者信息

Raine D A, Jeffrey I W, Clemens M J

机构信息

Department of Biochemistry, St George's Hospital Medical School, London, UK.

出版信息

FEBS Lett. 1998 Oct 9;436(3):343-8. doi: 10.1016/s0014-5793(98)01163-6.

DOI:10.1016/s0014-5793(98)01163-6
PMID:9801145
Abstract

Previous evidence has shown that the majority of the interferon-inducible, double-stranded RNA-dependent protein kinase PKR is associated with ribosomes in vivo. Here we show that ribosomes are inhibitory for PKR activity since they compete with dsRNA for binding to PKR, inhibit the activation of the protein kinase by dsRNA, and prevent the phosphorylation of the PKR substrate eIF2alpha. We suggest that ribosomes constitute a reservoir of inactive PKR and that the protein kinase must be displaced from the ribosome by dsRNA in order to become activated.

摘要

相似文献

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2
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引用本文的文献

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