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1
Rat 7,8-dihydro-8-oxoguanine DNA glycosylase: substrate specificity, kinetics and cleavagemechanism at an apurinic site.大鼠7,8-二氢-8-氧代鸟嘌呤DNA糖基化酶：底物特异性、动力学及无嘌呤位点的切割机制
Nucleic Acids Res. 1998 Nov 15;26(22):5199-202. doi: 10.1093/nar/26.22.5199.
2
Cloning and expression in Escherichia coli of the OGG1 gene of Saccharomyces cerevisiae, which codes for a DNA glycosylase that excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine.酿酒酵母OGG1基因在大肠杆菌中的克隆与表达，该基因编码一种DNA糖基化酶，可切除7,8-二氢-8-氧代鸟嘌呤和2,6-二氨基-4-羟基-5-N-甲基甲酰胺基嘧啶。
Proc Natl Acad Sci U S A. 1996 May 28;93(11):5197-202. doi: 10.1073/pnas.93.11.5197.
3
Distinct repair activities of human 7,8-dihydro-8-oxoguanine DNA glycosylase and formamidopyrimidine DNA glycosylase for formamidopyrimidine and 7,8-dihydro-8-oxoguanine.人7,8-二氢-8-氧代鸟嘌呤DNA糖基化酶和甲酰胺嘧啶DNA糖基化酶对甲酰胺嘧啶和7,8-二氢-8-氧代鸟嘌呤的不同修复活性
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4
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The C-terminal domain of MutY glycosylase determines the 7,8-dihydro-8-oxo-guanine specificity and is crucial for mutation avoidance.MutY糖基化酶的C末端结构域决定了7,8-二氢-8-氧代鸟嘌呤的特异性，对避免突变至关重要。
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The Ogg1 protein of Saccharomyces cerevisiae: a 7,8-dihydro-8-oxoguanine DNA glycosylase/AP lyase whose lysine 241 is a critical residue for catalytic activity.酿酒酵母的Ogg1蛋白：一种7,8-二氢-8-氧代鸟嘌呤DNA糖基化酶/AP裂解酶，其赖氨酸241是催化活性的关键残基。
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8
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9
Specific recognition of A/G and A/7,8-dihydro-8-oxoguanine (8-oxoG) mismatches by Escherichia coli MutY: removal of the C-terminal domain preferentially affects A/8-oxoG recognition.大肠杆菌MutY对A/G和A/7,8-二氢-8-氧代鸟嘌呤（8-氧代鸟嘌呤，8-oxoG）错配的特异性识别：C末端结构域的去除优先影响A/8-氧代鸟嘌呤的识别。
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10
Recognition and kinetics for excision of a base lesion within clustered DNA damage by the Escherichia coli proteins Fpg and Nth.大肠杆菌蛋白Fpg和Nth对簇状DNA损伤中碱基损伤的识别及切除动力学
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引用本文的文献

1
Limited repair of 8-hydroxy-7,8-dihydroguanine residues in human testicular cells.人类睾丸细胞中8-羟基-7,8-二氢鸟嘌呤残基的有限修复
Nucleic Acids Res. 2003 Feb 15;31(4):1351-63. doi: 10.1093/nar/gkg216.
2
Human DNA glycosylases of the bacterial Fpg/MutM superfamily: an alternative pathway for the repair of 8-oxoguanine and other oxidation products in DNA.细菌Fpg/MutM超家族的人类DNA糖基化酶：DNA中8-氧代鸟嘌呤和其他氧化产物修复的另一条途径。
Nucleic Acids Res. 2002 Nov 15;30(22):4926-36. doi: 10.1093/nar/gkf618.
3
Embryonic extracts derived from the nematode Caenorhabditis elegans remove uracil from DNA by the sequential action of uracil-DNA glycosylase and AP (apurinic/apyrimidinic) endonuclease.源自线虫秀丽隐杆线虫的胚胎提取物通过尿嘧啶-DNA糖基化酶和AP（无嘌呤/无嘧啶）内切核酸酶的顺序作用从DNA中去除尿嘧啶。
Biochem J. 2002 Jul 15;365(Pt 2):547-53. doi: 10.1042/BJ20020375.
4
Up-regulation of base excision repair activity for 8-hydroxy-2'-deoxyguanosine in the mouse brain after forebrain ischemia-reperfusion.前脑缺血再灌注后小鼠脑中8-羟基-2'-脱氧鸟苷碱基切除修复活性的上调。
J Neurochem. 2000 Mar;74(3):1098-105. doi: 10.1046/j.1471-4159.2000.741098.x.
5
Oxidative damage to the c-fos gene and reduction of its transcription after focal cerebral ischemia.局灶性脑缺血后c-fos基因的氧化损伤及其转录减少。
J Neurochem. 1999 Sep;73(3):1164-74. doi: 10.1046/j.1471-4159.1999.0731164.x.

大鼠7,8-二氢-8-氧代鸟嘌呤DNA糖基化酶：底物特异性、动力学及无嘌呤位点的切割机制

Rat 7,8-dihydro-8-oxoguanine DNA glycosylase: substrate specificity, kinetics and cleavagemechanism at an apurinic site.

作者信息

Prieto Alamo M J, Jurado J, Francastel E, Laval F

机构信息

Unité 347 INSERM, 80 Rue du Général Leclerc, 94276 Le Kremlin Bicêtre Cedex, France.

出版信息

Nucleic Acids Res. 1998 Nov 15;26(22):5199-202. doi: 10.1093/nar/26.22.5199.

DOI:10.1093/nar/26.22.5199
PMID:9801319
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC147981/
Abstract

Reactive oxygen species produce different lesions in DNA. Among them, 7,8-dihydro-8-oxoguanine (8-oxoG) is one of the major oxidative products implicated in mutagenesis. This lesion is removed from damaged DNA by base excision repair, and genes coding for 8-oxoG-DNA glycosylases have been isolated from bacteria, yeast and human cells. We have isolated and characterized the cDNA encoding the rat 8-oxoG-DNA glycosylase (rOGG1). Expression of the cDNA in the fgp mutY Escherichia coli double mutant allowed the purification of the untagged rOGG1 protein. It excises 8-oxoG from DNA with a strong preference for duplex DNA containing 8-oxoG:C base pairs. rOGG1 also acts on formamidopyrimidine (FaPy) residues, and the K m values on 8-oxoG and FaPy residues are 18.8 and 9.7 nM, respectively. When acting on an oligonucleotide containing an 8-oxoG residue, rOGG1 shows a beta-lyase activity that nicks DNA 3' to the lesion. However, rOGG1 acts on a substrate containing an apurinic site by a beta-delta elimination reaction and proceeds through a Schiff base intermediate. Expression of rOGG1 in E.coli fpg mutY suppresses its spontaneous mutator phenotype.

摘要

活性氧会在DNA中产生不同的损伤。其中，7,8 - 二氢 - 8 - 氧代鸟嘌呤（8 - 氧代鸟嘌呤，8 - oxoG）是与诱变作用相关的主要氧化产物之一。这种损伤通过碱基切除修复从受损DNA中去除，并且已经从细菌、酵母和人类细胞中分离出编码8 - 氧代鸟嘌呤 - DNA糖基化酶的基因。我们已经分离并鉴定了编码大鼠8 - 氧代鸟嘌呤 - DNA糖基化酶（rOGG1）的cDNA。该cDNA在fgp mutY大肠杆菌双突变体中的表达使得能够纯化未标记的rOGG1蛋白。它从DNA中切除8 - 氧代鸟嘌呤，对含有8 - 氧代鸟嘌呤：胞嘧啶碱基对的双链DNA有强烈偏好。rOGG1也作用于甲酰胺嘧啶（FaPy）残基，其对8 - 氧代鸟嘌呤和FaPy残基的K m值分别为18.8和9.7 nM。当作用于含有8 - 氧代鸟嘌呤残基的寡核苷酸时，rOGG1表现出β - 裂解酶活性，在损伤位点的3' 端切割DNA。然而，rOGG1通过β - δ消除反应作用于含有脱嘌呤位点的底物，并通过席夫碱中间体进行反应。rOGG1在大肠杆菌fpg mutY中的表达抑制了其自发的诱变表型。