一条反平行三链形成寡脱氧核糖核苷酸与人类α1（I）前胶原基因启动子形成三链螺旋。
Triple helix formation with the promoter of human alpha1(I) procollagen gene by an antiparallel triplex-forming oligodeoxyribonucleotide.
作者信息
Nakanishi M, Weber K T, Guntaka R V
机构信息
Department of Molecular Microbiology and Immunology and Division of Cardiology, School of Medicine, University of Missouri-Columbia, Columbia, MO 65212, USA.
出版信息
Nucleic Acids Res. 1998 Nov 15;26(22):5218-22. doi: 10.1093/nar/26.22.5218.
The promoters of alpha1(I) procollagen genes of vertebrates contain two contiguous stretches of polypyrimidine/polypurine sequences, referred to as C1 (-140 to -170) and C2 (-171 to -200). Antiparallel triplex-forming upstream oligonucleotides form efficient triplexes with C1. The C1 tract of human differs from rodent alpha1(I) promoters by 7 nt which are mainly A-->G transitions. Human triplex-forming oligodeoxyribonucleotide (TFO) formed stable triplexes efficiently with a K d of approximately 10-20 nM compared with a K d of approximately 100 nM for rodent TFO. Mutational analysis indicated that 3 or 4 nt (-153 to -155) are sufficient for this higher affinity. TFOs specific for human C1 inhibited transcription from human promoter both in vitro in HeLa cell nuclear extracts and in vivo in cultured chick embryo fibroblasts.
脊椎动物α1（I）前胶原基因的启动子包含两个连续的聚嘧啶/聚嘌呤序列片段，称为C1（-140至-170）和C2（-171至-200）。反平行三链体形成上游寡核苷酸与C1形成有效的三链体。人类的C1片段与啮齿动物α1（I）启动子在7个核苷酸上存在差异，主要是A→G转换。与啮齿动物三链体形成寡脱氧核糖核苷酸（TFO）的解离常数（Kd）约为100 nM相比，人类三链体形成寡脱氧核糖核苷酸（TFO）能有效地形成稳定的三链体，其Kd约为10-20 nM。突变分析表明，3或4个核苷酸（-153至-155）足以产生这种更高的亲和力。对人类C1特异的TFO在体外HeLa细胞核提取物和体内培养的鸡胚成纤维细胞中均抑制了来自人类启动子的转录。
Zotero 插件