Houtsmuller A B, Janssen P J, Nigg A L, Hoedemaeker R F, van der Kwast T H
Department of Pathology, Erasmus University of Rotterdam, The Netherlands.
Anal Quant Cytol Histol. 1998 Oct;20(5):365-72.
To evaluate androgen receptor (AR) levels to predict endocrine therapy response and prognosis.
A sepharose microsphere model was employed to establish the relationship between level of immunohistochemical staining density and both antigen and primary antibody (F39.4.1) concentration. Subsequently, the results of the model system were compared with the results in routine prostate sections.
A log-linear relationship was observed between optical density (OD) and primary antibody dilution measured in immunostained, antigen-coated microspheres with moderate antigen density. Similar titration curves were observed in prostate sections at the same dilution range, indicating that the microsphere model can be extrapolated to routine tissue sections. With microspheres with high coating density, the loglinear range of dilutions shifted to higher dilutions.
Differences in AR levels in prostate tissue sections might be more accurately detected by comparison of titration curves of primary antibody than by comparison of OD values at a fixed primary antibody dilution.
评估雄激素受体（AR）水平以预测内分泌治疗反应和预后。
采用琼脂糖微球模型建立免疫组织化学染色密度水平与抗原及一抗（F39.4.1）浓度之间的关系。随后，将模型系统的结果与常规前列腺切片的结果进行比较。
在具有中等抗原密度的免疫染色、抗原包被微球中，观察到光密度（OD）与一抗稀释度之间呈对数线性关系。在相同稀释范围内，前列腺切片中观察到类似的滴定曲线，表明微球模型可外推至常规组织切片。对于具有高包被密度的微球，稀释的对数线性范围向更高稀释度偏移。
与在固定一抗稀释度下比较OD值相比，通过比较一抗滴定曲线可能更准确地检测前列腺组织切片中AR水平的差异。
