Kimura N, Mizokami A, Oonuma T, Sasano H, Nagura H
Department of Pathology, Tohoku University School of Medicine, Sendai, Japan.
J Histochem Cytochem. 1993 May;41(5):671-8. doi: 10.1177/41.5.8468448.
We investigated the immunohistochemical localization of androgen receptor (AR) using a polyclonal antibody for 55 KD recombinant human AR in human tissues fixed with 4% paraformaldehyde solution and embedded in paraffin. Immunoreactive AR was restricted to the nuclei of various tissues. Among the well-known androgen target organs, secretory cells and basal cells of the prostate, spermatogonia, spermatocytes, Sertoli cells and Leydig cells of the testis, epithelial cells of the rete testis, fibroblasts in the whole organ, squamous cells, sweat gland and hair follicle cells of the skin, and hepatocytes of the liver were positive for AR. In addition, smooth muscle cells of the prostate, uterus, urinary bladder, gastrointestinal tract, arteries, and arterioles were strongly immunoreactive for AR. Cardiac muscle and striated muscle of psoas were positive for AR. Acinar cells, ductal cells, and myoepithelial cells of the breast, endocervical and endometrial cells of the uterus, cyto- and syncytiotrophoblast of the chorionic villi, and tubules of the kidney were also positive for AR. Most FSH, LH, and some GH endocrine cells in the anterior and posterior lobes of the pituitary gland, follicular cells of the thyroid gland, and adrenocortical cells were positive for AR. Cells immunoreactive for AR were ubiquitously distributed throughout the entire body. The present study demonstrated the diversity of androgen effects on many target tissues.
我们使用针对55KD重组人雄激素受体（AR）的多克隆抗体，对用4%多聚甲醛溶液固定并石蜡包埋的人体组织进行了AR的免疫组织化学定位研究。免疫反应性AR局限于各种组织的细胞核。在众所周知的雄激素靶器官中，前列腺的分泌细胞和基底细胞、睾丸的精原细胞、精母细胞、支持细胞和间质细胞、睾丸网的上皮细胞、整个器官的成纤维细胞、皮肤的鳞状细胞、汗腺和毛囊细胞以及肝脏的肝细胞AR呈阳性。此外，前列腺、子宫、膀胱、胃肠道、动脉和小动脉的平滑肌细胞AR免疫反应强烈。腰大肌的心肌和横纹肌AR呈阳性。乳腺的腺泡细胞、导管细胞和肌上皮细胞、子宫的宫颈内膜和子宫内膜细胞、绒毛膜绒毛的细胞滋养层和合体滋养层以及肾的小管AR也呈阳性。垂体前叶和后叶的大多数促卵泡激素（FSH）、促黄体生成素（LH）以及一些生长激素（GH）内分泌细胞、甲状腺的滤泡细胞和肾上腺皮质细胞AR呈阳性。AR免疫反应阳性细胞遍布全身。本研究证明了雄激素对许多靶组织作用的多样性。
