由杀菌剂代森锰锌诱导的慢生根瘤菌USDA 3187中的生化变化。

Biochemical alterations in Bradyrhizobium sp USDA 3187 induced by the fungicide Mancozeb.

作者信息

Fabra A, Angelini J, Donolo A, Permigiani M, Castro S

机构信息

Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Argentina.

出版信息

Antonie Van Leeuwenhoek. 1998 Apr;73(3):223-8. doi: 10.1023/a:1000987524112.

DOI:10.1023/a:1000987524112

PMID:9801766

Abstract

We have previously shown that fungicide Mancozeb causes a 50% decrease in Bradyhizobium sp USDA 3187 growth rate and affects the bacteria-root symbiotic interaction. In order to elucidate the fungicide toxicity mechanism we determined the effects of Mancozeb on cell chemical composition, glutathione (GSH) content (molecule involved in the detoxification process), glutathione S-transferase (GST) activity and on polyamine, exopolysaccharides, capsular polysaccharides and lipopolysaccharides. Mancozeb produced biochemical alterations in membrane composition, polysaccharides and polyamines. In spite of the increment of GSH content and GST activity, they are not enough to prevent the growth diminution.

摘要

我们之前已经表明，杀菌剂代森锰锌会使慢生根瘤菌属USDA 3187的生长速率降低50%，并影响细菌与根的共生相互作用。为了阐明杀菌剂的毒性机制，我们测定了代森锰锌对细胞化学成分、谷胱甘肽（GSH）含量（参与解毒过程的分子）、谷胱甘肽S-转移酶（GST）活性以及对多胺、胞外多糖、荚膜多糖和脂多糖的影响。代森锰锌在膜成分、多糖和多胺方面产生了生化改变。尽管GSH含量和GST活性有所增加，但它们不足以阻止生长的减少。