Horáková K, Fusková A, Ceglédyová N
Neoplasma. 1976;23(5):499-505.
Optimal conditions were determined for the distribution of Ehrlich ascites carcinoma (EAC) and L-5178Y mouse tumor cells, proliferating in vivo, by their age within the cell cycle by sedimentation in a buffered linear sacharose density gradient. Measurements of cell size, DNA content and incorporation of tritiated thymidine in successive parts of the gradient confirmed the actual separation of cells of different age: in the upper fractions there were cells in G1 phase, in the middle fractions in S phase and in the lower layers of the gradient there were cells in G2 and/or M phase.
通过在缓冲线性蔗糖密度梯度中沉降，确定了在体内增殖的艾氏腹水癌（EAC）和L-5178Y小鼠肿瘤细胞按其在细胞周期中的年龄分布的最佳条件。对梯度连续部分中的细胞大小、DNA含量和氚标记胸腺嘧啶核苷掺入的测量证实了不同年龄细胞的实际分离：在梯度上部有处于G1期的细胞，中部有处于S期的细胞，梯度下层有处于G2和/或M期的细胞。
