Kaneko H, Fujikawa T, Alam K S, Kaneko S, Tanaka M, Hibasami H, Nakashima K
Department of Biochemistry, Mie University Faculty of Medicine, Japan.
Biochem Mol Biol Int. 1998 Oct;46(2):411-4. doi: 10.1080/15216549800203922.
The cyclin D1/PRAD1 gene is correlated with carcinogenesis of human breast cancer. In this study, we have analyzed effects of breast cancer-related hormones on the cyclin D1 gene expression in T-47D human breast cancer cells. Estradiol (E2) and human prolactin (hPRL) equally enhanced the cyclin D1 gene expression in the cells, and 22 and 20 kDa human growth hormones (22K and 20K hGHs) showed less stimulatory effects. In the presence of E2, however, hPRL or 22K hGH showed additive stimulations of the cyclin D1 gene expression to that by E2 alone, while 20K hGH did not show any additive stimulation of the gene expression. The results suggest that the signal pathways through estrogen and hPRL receptors are important for cyclin D1 gene expression in breast cancer cells, and that 20K hGH has little effect on the cyclin D1 gene expression in these cells because of its lower affinity to PRL receptor.
细胞周期蛋白D1/PRAD1基因与人类乳腺癌的发生相关。在本研究中，我们分析了乳腺癌相关激素对T-47D人乳腺癌细胞中细胞周期蛋白D1基因表达的影响。雌二醇（E2）和人催乳素（hPRL）同样增强了细胞中细胞周期蛋白D1基因的表达，而22 kDa和20 kDa的人生长激素（22K和20K hGHs）的刺激作用较小。然而，在E2存在的情况下，hPRL或22K hGH对细胞周期蛋白D1基因表达的刺激作用与单独使用E2时具有加和性，而20K hGH对基因表达没有任何加和性刺激作用。结果表明，通过雌激素和hPRL受体的信号通路对乳腺癌细胞中细胞周期蛋白D1基因表达很重要，并且20K hGH对这些细胞中细胞周期蛋白D1基因表达的影响很小，因为它对PRL受体的亲和力较低。
