Venturini G, Fioravanti E, Colasanti M, Persichini T, Ascenzi P
Department of Biology, University of Rome Tre, Italy.
Biochem Mol Biol Int. 1998 Oct;46(2):425-8. doi: 10.1080/15216549800203942.
Nitric oxide (NO) may modulate the catalytic activity of cysteine proteases. In the present study, the inhibitory effect of NO, released by the NO-donors (+/-)-(E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide and nitroprusside, on papain action is reported. Papain inactivation via NO-mediated nitrosylation of the Cys25 catalytic residue represents a molecular model for cysteine protease inhibition.
一氧化氮（NO）可能会调节半胱氨酸蛋白酶的催化活性。在本研究中，报告了由NO供体（±）-（E）-4-乙基-2-[(E)-羟基亚氨基]-5-硝基-3-己烯酰胺和硝普钠释放的NO对木瓜蛋白酶作用的抑制效果。通过Cys25催化残基的NO介导的亚硝基化使木瓜蛋白酶失活，这代表了一种半胱氨酸蛋白酶抑制的分子模型。
