Busscher H J, Bruinsma G, van Weissenbruch R, Leunisse C, van der Mei H C, Dijk F, Albers F W
Laboratory for Materia Technica, University of Groningen, The Netherlands.
Eur Arch Otorhinolaryngol. 1998;255(8):410-3. doi: 10.1007/s004050050088.
Biofilm formation on indwelling silicone rubber voice prostheses in laryngectomized patients is still the main reason for dysfunction of the valve, leading to frequent replacements. Within patient support groups in The Netherlands, laryngectomees have suggested that the consumption of buttermilk prolongs the life-time of indwelling silicone rubber voice prostheses. The aim of the present study was to compare biofilm formation on Groningen button voice prostheses in a so-called artificial throat. Ten prostheses were placed in a simulated control group and ten other prostheses in a group with a simulated consumption of 700 ml buttermilk three times a day. Biofilms were allowed to grow on the prostheses by inoculating two artificial throats with the total cultivable microflora (bacteria and yeasts) isolated from an explanted Groningen button voice prosthesis. After 3 days, one artificial throat was perfused three times daily with phosphate buffer (control group) for 8 days, while the other artificial throat was perfused with buttermilk. Prostheses removed from the artificial throat in the control group were covered with a thick biofilm. Scanning electron microscopy showed microcolonies growing into the silicone rubber, similar to the ingrowth observed on explanted Groningen buttons. The simulated consumption of buttermilk in the other artificial throat almost fully prevented the formation of a biofilm on the prostheses during the experimental period. These in vitro experiments in the artificial throat demonstrate that the deterioration of voice prostheses can be lessened by the daily intake of buttermilk through its inhibitory effects on biofilm formation.
喉切除患者体内留置的硅橡胶语音假体上生物膜的形成仍然是瓣膜功能障碍的主要原因，导致频繁更换。在荷兰的患者支持小组中，喉切除患者建议饮用酪乳可延长体内留置硅橡胶语音假体的使用寿命。本研究的目的是比较在所谓的人工喉中格罗宁根纽扣式语音假体上生物膜的形成情况。将10个假体置于模拟对照组，另外10个假体置于模拟每天饮用700毫升酪乳三次的组中。通过用从一个取出的格罗宁根纽扣式语音假体中分离出的可培养微生物群落（细菌和酵母）接种两个人工喉，使生物膜在假体上生长。3天后，一个人工喉每天用磷酸盐缓冲液灌注三次（对照组），持续8天，而另一个人工喉用酪乳灌注。从对照组人工喉中取出的假体覆盖着一层厚厚的生物膜。扫描电子显微镜显示微菌落生长到硅橡胶中，类似于在取出的格罗宁根纽扣上观察到的向内生长。在另一个人工喉中模拟饮用酪乳在实验期间几乎完全防止了假体上生物膜的形成。在人工喉中进行的这些体外实验表明，通过酪乳对生物膜形成的抑制作用，每天摄入酪乳可减轻语音假体的退化。
