健康犬和患有马拉色菌性皮炎的犬对厚皮马拉色菌的体液免疫和细胞介导免疫反应。

Humoral and cell-mediated responses to Malassezia pachydermatis in healthy dogs and dogs with Malassezia dermatitis.

作者信息

Bond R, Elwood C M, Littler R M, Pinter L, Lloyd D H

机构信息

Department of Small Animal Medicine and Surgery, Royal Veterinary College, University of London, North Mymms, Hatfield.

出版信息

Vet Rec. 1998 Oct 3;143(14):381-4. doi: 10.1136/vr.143.14.381.

DOI:10.1136/vr.143.14.381

PMID:9802194

Abstract

The in vitro proliferative responses of peripheral blood mononuclear cells from seven healthy basset hounds exposed to Malassezia pachydermatis antigen (500 micrograms/ml) exceeded (P < 0.05) those of seborrhoeic basset hounds with high populations of M pachydermatis and eight Irish setters with gluten-sensitive enteropathy. The stimulation indices in the latter two groups and in eight healthy beagles were comparable. The stimulation indices of the four groups after exposure to phytohaemaglutinin did not differ significantly. The serum titres of M pachydermatis-specific IgG and IgA measured by enzyme-linked immunosorbent assays (ELISA) in 21 seborrhoeic basset hounds and 11 affected dogs of various breeds exceeded those of 14 healthy basset hounds and eight healthy beagles (P < 0.01 for IgG, P < 0.05 for IgA). Total serum IgA concentrations measured by ELISA in the affected dogs were not lower than those of healthy dogs.

摘要

七只健康巴吉度猎犬的外周血单个核细胞在暴露于厚皮马拉色菌抗原（500微克/毫升）后的体外增殖反应超过了（P < 0.05）厚皮马拉色菌数量多的脂溢性皮炎巴吉度猎犬以及八只患有麸质敏感肠病的爱尔兰赛特犬。后两组以及八只健康比格犬的刺激指数相当。四组在暴露于植物血凝素后的刺激指数无显著差异。通过酶联免疫吸附测定（ELISA）在21只脂溢性皮炎巴吉度猎犬和11只不同品种患病犬中测得的厚皮马拉色菌特异性IgG和IgA血清滴度超过了14只健康巴吉度猎犬和八只健康比格犬（IgG为P < 0.01，IgA为P < 0.05）。通过ELISA在患病犬中测得的总血清IgA浓度不低于健康犬。