Fung E T, Lanahan A, Worley P, Huganir R L
Department of Neuroscience, Howard Hughes Medical Institute, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
FEBS Lett. 1998 Oct 16;437(1-2):29-33. doi: 10.1016/s0014-5793(98)01151-x.
Nicotinic acetylcholine receptors (nAChRs) are initially expressed diffusely on the surface of myotubes and, in response to neuronally derived factors, cluster at the endplate to a final concentration of approximately 10000/microm2. The synaptic peripheral membrane protein rapsyn has been shown to mediate clustering of nAChRs in several systems. Here we describe the use of the yeast two-hybrid system to identify proteins that can interact with rapsyn. One of the clones we have identified is a Torpedo californica homolog of the Src-associated in mitosis protein (Sam68). We further show that Sam68, like rapsyn, is localized at the neuromuscular junction.
烟碱型乙酰胆碱受体（nAChRs）最初在肌管表面呈弥散性表达，并且在神经元衍生因子的作用下，在终板处聚集，最终浓度约为10000/μm²。突触外周膜蛋白rapsyn已被证明在多个系统中介导nAChRs的聚集。在这里，我们描述了使用酵母双杂交系统来鉴定能够与rapsyn相互作用的蛋白质。我们鉴定出的其中一个克隆是有丝分裂相关的Src结合蛋白（Sam68）的加州电鳐同源物。我们进一步表明，Sam68与rapsyn一样，定位于神经肌肉接头处。
