Yanagi K, Miyoshi H, Ohshima N
Department of Biomedical Engineering, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba Science City, Japan.
ASAIO J. 1998 Sep-Oct;44(5):M436-40. doi: 10.1097/00002480-199809000-00022.
A packed-bed reactor using reticulated polyvinyl formal (PVF) resin as a support material is a useful configuration to achieve high density culture of hepatocytes for use as a bioartificial liver. The authors investigated the effects of oxygen concentrations of the culture medium on the metabolic performance of hepatocytes cultured in the reactor. A packed-bed reactor loaded with 250 PVF resin cubes (2 x 2 x 2 mm) was used. Hepatocytes obtained from male Wistar rats were inoculated into the reactor. Culture medium was perfused from the reservoir into the reactor through an oxygenator using a roller pump. Concentration of the dissolved oxygen in the medium was controlled by changing the gas mixture ratio supplied to the oxygenator. Hepatocytes cultured in the packed-bed reactor (cell density: 8.6 x 10(6) cells/cm3 PVF) under conditions of high dissolved oxygen concentrations ranging from 260 to 460 micromol/L showed 30% higher ammonium metabolic activity and 85% higher albumin secretion activity compared with those from the monolayer culture in the earlier culture stage (up to 2 days). However, low oxygen concentrations in the medium (<100 micromol/L) impaired activities of cultured hepatocytes.
使用网状聚乙烯醇缩甲醛（PVF）树脂作为支撑材料的填充床反应器是实现用于生物人工肝的肝细胞高密度培养的一种有用配置。作者研究了培养基中氧气浓度对在该反应器中培养的肝细胞代谢性能的影响。使用了一个装有250个PVF树脂立方体（2×2×2毫米）的填充床反应器。从雄性Wistar大鼠获得的肝细胞被接种到反应器中。培养基通过蠕动泵从储液器经充氧器灌注到反应器中。通过改变供应到充氧器的气体混合比来控制培养基中溶解氧的浓度。在260至460微摩尔/升的高溶解氧浓度条件下，在填充床反应器中培养的肝细胞（细胞密度：8.6×10⁶个细胞/立方厘米PVF）与早期培养阶段（长达2天）的单层培养相比，铵代谢活性高30%，白蛋白分泌活性高85%。然而，培养基中的低氧浓度（<100微摩尔/升）会损害培养肝细胞的活性。
