Ni A, Chai K X, Chao L, Chao J
Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA.
Biochim Biophys Acta. 1998 Nov 8;1442(2-3):177-85. doi: 10.1016/s0167-4781(98)00163-8.
The gene encoding rat bradykinin B1 receptor has been cloned by using a partial rat B1 cDNA probe. The rat B1 receptor gene contains two exons and the entire coding region is within the second exon. The 5'-flanking region of the rat B1 receptor gene contains several putative transcriptional regulatory sites including TATA box, cAMP response element, NF-kappaB and AP-1. It showed promoter activity inducible by lipopolysaccharide in vascular smooth muscle cells. Rat B1 receptor mRNA was found to be alternatively spliced and induced by lipopolysaccharide treatment in a wide range of tissues, such as the salivary gland, testis, kidney, lung, heart, prostate and aorta. The deduced rat B1 receptor amino acid sequence is 71% homologous to human and rabbit counterparts, and 89% homologous to the mouse counterpart. The expressed B1 receptor in HEK293 cells displayed a rank order of affinity for the kinin peptides: des-Arg9-BK>Lys-des-Arg9-BK approximately des-Arg9, Leu8-BK>Sar-Tyr-epsilonAhx-Lys-[D-betaNal7, Ile8]-des-Arg9-BK>Sar-Tyr-epsilonAhx-Lys-des-Arg9-BK>>BK>> Hoe140. These results indicate that the cloned gene encodes a functional rat B1 receptor.
利用大鼠缓激肽B1 cDNA探针的一部分克隆了编码大鼠缓激肽B1受体的基因。大鼠B1受体基因包含两个外显子，整个编码区位于第二个外显子内。大鼠B1受体基因的5'侧翼区包含几个假定的转录调控位点，包括TATA盒、cAMP反应元件、NF-κB和AP-1。它在血管平滑肌细胞中显示出脂多糖诱导的启动子活性。发现大鼠B1受体mRNA可选择性剪接，并在多种组织如唾液腺、睾丸、肾脏、肺、心脏、前列腺和主动脉中经脂多糖处理后诱导产生。推导的大鼠B1受体氨基酸序列与人和兔的相应序列同源性为71%，与小鼠的相应序列同源性为89%。在HEK293细胞中表达的B1受体对激肽肽的亲和力顺序为：去-Arg9-BK>Lys-去-Arg9-BK≈去-Arg9, Leu8-BK>Sar-Tyr-εAhx-Lys-[D-βNal7, Ile8]-去-Arg9-BK>Sar-Tyr-εAhx-Lys-去-Arg9-BK>>BK>>Hoe140。这些结果表明克隆的基因编码功能性大鼠B1受体。
