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[杂交菌落技术在产毒霍乱弧菌01型鉴定中的应用]

[The application of the hybridization in colonies technic for the identification of toxigenic Vibrio cholerae 01].

作者信息

Bravo Fariñas L, Monté Boada R J, Ramírez Alvarez M, Maestre Mesa J L, Suárez Moreno O, Morales Grillo J

机构信息

Instituto de Medicina Tropical Pedro Kourí.

出版信息

Rev Cubana Med Trop. 1996;48(3):169-70.

PMID:9805044
Abstract

By means of the polymerase chain reaction (PCR) it was obtained a probe for the gen that codifies the subunit B of cholerae toxin (CTxB), which carried a Vibrio cholerae 01 reference strain. The checking of the amplified product was performed by using the hybridization techniques in colonies. This product hybridized with the gen that codifies for the subunit B of cholerae toxin isolated from Peru and Ecuador, representing the present epidemics in Latin America, but it did not so with the phylogenetically related strains.

摘要

通过聚合酶链反应(PCR),获得了编码霍乱毒素B亚基(CTxB)基因的探针,该探针携带霍乱弧菌O1参考菌株。通过菌落杂交技术对扩增产物进行检测。该产物与从秘鲁和厄瓜多尔分离出的编码霍乱毒素B亚基的基因杂交,这些基因代表了拉丁美洲目前的疫情,但与系统发育相关菌株的基因不杂交。

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