Karunasagar I, Sugumar G, Karunasagar I, Reilley A
Department of Fishery Microbiology, University of Agricultural Sciences, College of Fisheries, Mangalore, India.
Mol Mar Biol Biotechnol. 1995 Dec;4(4):365-8.
The possibility of detecting Vibrio cholerae contamination of seafood using a technique based on polymerase chain reaction (PCR) was studied. Direct PCR on lysate prepared from fish homogenates containing 10(3) V. cholerae/ml gave a positive reaction. When combined with alkaline peptone water (APW) enrichment, homogenates containing 1.4 cells/ml gave amplification signal. The technique could also detect V. cholerae O139, the recent epidemic serotype in the Indian subcontinent. An environmental isolate of non-O1 V. cholerae that produced cholera toxin was also positive in this assay. The results suggest that PCR-based techniques have great potential in quick detection of toxigenic V. cholerae in seafoods.
研究了使用基于聚合酶链反应（PCR）的技术检测海鲜中霍乱弧菌污染的可能性。对含有每毫升10³霍乱弧菌的鱼匀浆制备的裂解物进行直接PCR，得到了阳性反应。当与碱性蛋白胨水（APW）富集相结合时，每毫升含有1.4个细胞的匀浆产生了扩增信号。该技术还可以检测霍乱弧菌O139，这是印度次大陆最近流行的血清型。一株产生霍乱毒素的非O1群霍乱弧菌环境分离株在该检测中也呈阳性。结果表明，基于PCR的技术在快速检测海鲜中产毒霍乱弧菌方面具有巨大潜力。
