Mederos Cuervo L M, Ferrá Salazar C, Jimenez Misas C A, Valdivia Alvarez J A
Instituto de Medicina Tropical Pedro Kourí.
Rev Cubana Med Trop. 1995;47(1):54-8.
The macrobroth dilution method was used for determining the resistance and/or sensitivity of 10 nontuberculous mycobacteria strains from Mycobacterium avium-intracellulare (MAI) complex isolated from patients presenting with respiratory symptoms. Drugs employed were as follows: isoniazid, ethambutol, rifampicin, streptomycin, gentamicin and amikacin. With respect to susceptibility of the strains studied, the greater sensitivity was found to be against ethambutol, amikacin, and gentamicin. The possibility of replacing the Middlebrook 7H9 liquid culture medium by the UIT-L liquid culture medium was analyzed. Normalization of the method was attained and a shortening of the reading time when using the UIT-L culture medium was also obtained.
采用常量肉汤稀释法测定了从有呼吸道症状患者中分离出的10株鸟分枝杆菌-胞内分枝杆菌（MAI）复合群非结核分枝杆菌菌株的耐药性和/或敏感性。使用的药物如下：异烟肼、乙胺丁醇、利福平、链霉素、庆大霉素和阿米卡星。就所研究菌株的敏感性而言，发现对乙胺丁醇、阿米卡星和庆大霉素的敏感性更高。分析了用UIT-L液体培养基替代Middlebrook 7H9液体培养基的可能性。实现了该方法的标准化，并且在使用UIT-L培养基时还缩短了读数时间。
