Chaturvedi R, Agarkar V, Sharma G L, Sarma P U
Centre for Biochemical Technology, Delhi, India.
Prep Biochem Biotechnol. 1998 Nov;28(4):293-303. doi: 10.1080/10826069808010142.
Alpha Fetoprotein (AFP) is a major serum protein in the developing fetus and is of clinical significance as it is an oncofetal protein being synthesized by fetal organs and malignant tumors. AFP is here used as a diagnostic marker for hepatic carcinomas. In view of structural homology and similarities in physico-chemical properties with serum albumin, the separation and purification of AFP has always been a problem. Immunologically active AFP has been purified from human cord plasma using pseudoaffinity chromatography based on Cibacron blue substituted Sephadex G-100. AFP was quantified using rocket immunoelectrophoresis and double sandwich ELISA. Polyclonal antibodies were raised against purified AFP in mice. The purified antibodies were conjugated with peroxidase for use in double antibody sandwich ELISA. Purification of AFP from human cord plasma by an improved method with 55% recovery is reported.
甲胎蛋白（AFP）是发育中胎儿的一种主要血清蛋白，作为一种由胎儿器官和恶性肿瘤合成的癌胚蛋白具有临床意义。AFP在此用作肝癌的诊断标志物。鉴于其与血清白蛋白在结构同源性和物理化学性质上的相似性，AFP的分离和纯化一直是个问题。基于Cibacron blue取代的葡聚糖凝胶G - 100，利用假亲和色谱法从人脐带血浆中纯化出了具有免疫活性的AFP。使用火箭免疫电泳和双夹心ELISA对AFP进行定量。用纯化的AFP在小鼠体内制备多克隆抗体。将纯化的抗体与过氧化物酶偶联用于双抗体夹心ELISA。报道了一种改进方法从人脐带血浆中纯化AFP，回收率为55%。
