Afchain D, Fruit J, Yarzabal L, Capron A
Am J Trop Med Hyg. 1978 May;27(3):478-82. doi: 10.4269/ajtmh.1978.27.478.
A monospecific rabbit antiserum was prepared against a specific component of Tryanosoma cruzi epimastigote culture forms (designated antigen 5). Rabbits were immunized intradermally in 30-50 sites with the zone of agarose gel in which the component 5:anti-5 complex was visualized after two-dimensional immunoelectrophoresis. With this appropriate antiserum and the total epimastigote soluble extracts, the specific component of T. cruzi was purified by immunoadsorption. Purity and specificity controls of thefinal product were carried out by prolonged immunization of rabbits, and then by immunoelectrophoretic and two-dimensional electrophoretic analyses.
制备了一种针对克氏锥虫上鞭毛体培养形式的特定成分（命名为抗原5）的单特异性兔抗血清。将二维免疫电泳后可见成分5:抗5复合物的琼脂糖凝胶区域在30 - 50个部位皮内注射免疫兔子。用这种合适的抗血清和上鞭毛体总可溶性提取物，通过免疫吸附法纯化克氏锥虫的特定成分。通过对兔子进行长期免疫，然后进行免疫电泳和二维电泳分析，对最终产物进行纯度和特异性控制。
