Huang W, Feaver W J, Tomkinson A E, Friedberg E C
Department of Pathology, University of Texas, Southwestern Medical Center, Dallas 75235-9072, USA.
Mutat Res. 1998 Sep 11;408(3):183-94. doi: 10.1016/s0921-8777(98)00031-7.
Nucleotide excision repair (NER) of DNA in the yeast Saccharomyces cerevisiae and in human cells has been shown to be a biochemically complex process involving multiple gene products. In yeast, the involvement of the DNA replication accessory proteins, replication protein A (RPA1) and proliferating cell nuclear antigen (PCNA) in NER has not been demonstrated genetically. In this study we have generated temperature-degradable rfa1 and pcna mutants and show that these mutants are defective in NER in vitro under conditions that promote degradation of the RFA1 and PCNA gene products. We also demonstrate a physical interaction between RPA1 protein and subunits of the RNA polymerase II basal transcription factor IIH (TFIIH).
在酿酒酵母和人类细胞中，DNA的核苷酸切除修复（NER）已被证明是一个涉及多种基因产物的生物化学复杂过程。在酵母中，DNA复制辅助蛋白复制蛋白A（RPA1）和增殖细胞核抗原（PCNA）参与核苷酸切除修复尚未得到遗传学证明。在本研究中，我们构建了温度可降解的rfa1和pcna突变体，并表明在促进RFA1和PCNA基因产物降解的条件下，这些突变体在体外核苷酸切除修复中存在缺陷。我们还证明了RPA1蛋白与RNA聚合酶II基础转录因子IIH（TFIIH）亚基之间存在物理相互作用。
