酵母自主复制序列结合因子参与核苷酸切除修复。
Yeast autonomously replicating sequence binding factor is involved in nucleotide excision repair.
作者信息
Reed S H, Akiyama M, Stillman B, Friedberg E C
机构信息
Laboratory of Molecular Pathology, Department of Pathology, University of Texas Southwestern Medical Center, Dallas, Texas 75235, USA.
出版信息
Genes Dev. 1999 Dec 1;13(23):3052-8. doi: 10.1101/gad.13.23.3052.
Abstract
Nucleotide excision repair (NER) in yeast is effected by the concerted action of a large complex of proteins. Recently, we identified a stable subcomplex containing the yeast Rad7 and Rad16 proteins. Here, we report the identification of autonomously replicating sequence binding factor 1 (ABF1) as a component of the Rad7/Rad16 NER subcomplex. Yeast ABF1 protein is encoded by an essential gene required for DNA replication, transcriptional regulation, and gene silencing. We show that ABF1 plays a direct role in NER in vitro. Additionally, consistent with a role of ABF1 protein in NER in vivo, we show that certain temperature-sensitive abf1 mutant strains that are defective in DNA replication are specifically defective in the removal of photoproducts by NER and are sensitive to killing by ultraviolet (UV) radiation. These studies define a novel and unexpected role for ABF1 protein during NER in yeast.
摘要
酵母中的核苷酸切除修复（NER）是由一大组蛋白质协同作用实现的。最近，我们鉴定出了一个包含酵母Rad7和Rad16蛋白的稳定亚复合物。在此，我们报告自主复制序列结合因子1（ABF1）作为Rad7/Rad16 NER亚复合物的一个组分被鉴定出来。酵母ABF1蛋白由DNA复制、转录调控和基因沉默所需的一个必需基因编码。我们表明ABF1在体外NER中起直接作用。此外，与ABF1蛋白在体内NER中的作用一致，我们表明某些在DNA复制方面有缺陷的温度敏感型abf1突变菌株在通过NER去除光产物方面存在特异性缺陷，并且对紫外线（UV）辐射杀伤敏感。这些研究确定了ABF1蛋白在酵母NER过程中的一种新的意外作用。
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本文引用的文献
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The N-degron protein degradation strategy for investigating the function of essential genes: requirement for replication protein A and proliferating cell nuclear antigen proteins for nucleotide excision repair in yeast extracts.用于研究必需基因功能的N-端规则蛋白降解策略：酵母提取物中核苷酸切除修复对复制蛋白A和增殖细胞核抗原蛋白的需求
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The yeast RAD7 and RAD16 genes are required for postincision events during nucleotide excision repair. In vitro and in vivo studies with rad7 and rad16 mutants and purification of a Rad7/Rad16-containing protein complex.酵母RAD7和RAD16基因是核苷酸切除修复过程中切口后事件所必需的。对rad7和rad16突变体进行的体外和体内研究以及对含Rad7/Rad16蛋白复合物的纯化。
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