Goto T, Brunette D M
Faculty of Dentistry, The University of British Columbia, 2199 Wesbrook Mall, Vancouver, British Columbia, V6T 1Z3, Canada.
Exp Cell Res. 1998 Nov 1;244(2):474-80. doi: 10.1006/excr.1998.4196.
Tenascin is an extracellular matrix glycoprotein which affects cell behavior such as cell migration. This study was undertaken to investigate the time of appearance of tenascin (TN) in human gingival fibroblasts (HGF) and how it was affected by the surface topography of the titanium substratum or by serum concentration in the medium. HGF were cultured for 4 to 24 h and then processed for confocal immunofluorescence microscopy. Very few cells stained positive for TN 4 h after plating, but the number of TN-positive HGF gradually increased between 8 and 18 h after plating. The increase in the rate of the proportion of TN-positive cells on the grooved surface lagged behind that of HGF cultured on the smooth surface. The number of TN-positive cells in medium + 15% serum was significantly greater than that of cells in 5% serum or serum-free medium. The number of TN-positive cells was greater on the smooth titanium surface than on the grooved titanium surface in both 15% serum and 5% serum-containing medium. These findings suggest that TN production by fibroblasts in vitro can be modulated by factors in serum and by the surface topography of the substratum.
腱生蛋白是一种细胞外基质糖蛋白，它会影响细胞行为，如细胞迁移。本研究旨在调查腱生蛋白（TN）在人牙龈成纤维细胞（HGF）中出现的时间，以及它如何受到钛基质表面形貌或培养基中血清浓度的影响。将HGF培养4至24小时，然后进行共聚焦免疫荧光显微镜检查。接种后4小时，很少有细胞TN染色呈阳性，但接种后8至18小时，TN阳性HGF的数量逐渐增加。在有凹槽表面上TN阳性细胞比例的增加速率落后于在光滑表面上培养的HGF。培养基中含15%血清时TN阳性细胞的数量显著多于含5%血清或无血清培养基中的细胞数量。在含15%血清和含5%血清的培养基中，光滑钛表面上TN阳性细胞的数量均多于有凹槽钛表面。这些发现表明，体外成纤维细胞产生TN可受到血清中的因素和基质表面形貌的调节。
