萤火虫荧光素酶有两个核苷酸结合位点：单磷酸核苷和辅酶A对发光光谱的影响。
Firefly luciferase has two nucleotide binding sites: effect of nucleoside monophosphate and CoA on the light-emission spectra.
作者信息
Steghens J P, Min K L, Bernengo J C
机构信息
Laboratoire de Biochimie C, Hôpital Edouard Herriot, place d'Arsonval, 69437 Lyon Cedex 3, France.
出版信息
Biochem J. 1998 Nov 15;336 ( Pt 1)(Pt 1):109-13. doi: 10.1042/bj3360109.
Abstract
A laboratory-made spectroluminometer was used to analyse the light emitted by firefly (Photinus pyralis) luciferase reacting with several nucleotide derivatives. The analysis of the light emission in the presence of ATP or dATP provides some evidence that the enzyme has two nucleotide binding sites, each one leading to the formation of a complex emitting mainly at 575 nm (ATP) or 610 nm (dATP). AMP is able to displace dATP from the second site (610 nm) to the first one. Photoaffinity labelling of the second site by 8-azido-AMP gives similar results. The amplification effect of CoA and acetyl-CoA is also reconsidered according to this model.
摘要
使用实验室自制的分光光度计分析萤火虫（Photinus pyralis）荧光素酶与几种核苷酸衍生物反应所发出的光。在ATP或dATP存在下对发光的分析提供了一些证据，表明该酶有两个核苷酸结合位点，每个位点都会导致形成主要在575nm（ATP）或610nm（dATP）处发光的复合物。AMP能够将dATP从第二个位点（610nm）置换到第一个位点。用8-叠氮基-AMP对第二个位点进行光亲和标记得到了类似的结果。根据该模型，还重新考虑了辅酶A和乙酰辅酶A的放大作用。
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