Huang G T, Haake S K, Kim J W, Park N H
Section of Biology, UCLA School of Dentistry 90095-1668, USA.
Oral Microbiol Immunol. 1998 Oct;13(5):301-9. doi: 10.1111/j.1399-302x.1998.tb00711.x.
Little is known regarding the molecules expressed by gingival epithelial cells that are involved in initiating and maintaining inflammation following the interaction with periodontal pathogens. Thus, we investigated the effect of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis infection on the expression of neutrophil chemoattractant interleukin 8 (IL-8) and the adhesion molecule intercellular adhesion molecule-1 by gingival epithelial cells. The data revealed that both IL-8 and intercellular adhesion molecule-1 expression increased after infection with A. actinomycetemcomitans (IL-8: 2- to 7-fold; intercellular adhesion molecule-1: 2.5- to 3.7-fold). IL-8 secretion reached a maximal level 6 h after the infection and the expression subsequently decreased to basal level. The increased cell surface intercellular adhesion molecule-1 expression started at 4 h after infection and reached a maximal level 14 h after the infection. In contrast, the expression of both molecules rapidly decreased 2 h after challenge with P. gingivalis. This opposite influence of A. actinomycetemcomitans and P. gingivalis infection on the expression of IL-8 and intercellular adhesion molecule-1 by gingival epithelial cells suggests that A. actinomycetemcomitans infection may initiate the recruitment of neutrophils, whereas the P. gingivalis infection may retard this process and therefore demonstrate a distinct perspective of virulence.
关于牙龈上皮细胞在与牙周病原体相互作用后启动和维持炎症过程中所表达的分子，我们了解甚少。因此，我们研究了伴放线放线杆菌和牙龈卟啉单胞菌感染对牙龈上皮细胞中性粒细胞趋化因子白细胞介素8（IL-8）和黏附分子细胞间黏附分子-1表达的影响。数据显示，感染伴放线放线杆菌后，IL-8和细胞间黏附分子-1的表达均增加（IL-8：2至7倍；细胞间黏附分子-1：2.5至3.7倍）。感染后6小时，IL-8分泌达到最高水平，随后表达降至基础水平。感染后4小时，细胞表面细胞间黏附分子-1表达开始增加，感染后14小时达到最高水平。相反，用牙龈卟啉单胞菌攻击后2小时，这两种分子的表达迅速下降。伴放线放线杆菌和牙龈卟啉单胞菌感染对牙龈上皮细胞IL-8和细胞间黏附分子-1表达的这种相反影响表明，伴放线放线杆菌感染可能启动中性粒细胞的募集，而牙龈卟啉单胞菌感染可能会阻碍这一过程，因此显示出不同的毒力视角。
