Yang F, Quan J, Zhang T, Ito Y
Beijing Institute of New Technology Application, China.
J Chromatogr A. 1998 Oct 2;822(2):316-20. doi: 10.1016/s0021-9673(98)00624-4.
pH-Zone-refining counter-current chromatography was applied to the separation of alkaloids from a crude extract of the root of Sophora flavescens Ait using a multilayer coil planet centrifuge. After methyl tert.-butyl ether and water were equilibrated, triethylamine (10 mM) was added to the organic phase as a retainer and hydrochloric acid (5-10 mM) to the aqueous phase as an eluter. The separation was performed by eluting the aqueous phase while the organic phase was used as the stationary phase. From 1.0 g of the crude extract, sophocarpine (170 mg) and matrine (600 mg) were separated within 4.5 h at high purity of over 98%.
采用多层线圈行星离心机，运用pH区带精制逆流色谱法从苦参根粗提物中分离生物碱。甲基叔丁基醚和水达到平衡后，向有机相中加入三乙胺（10 mM）作为保留剂，向水相中加入盐酸（5 - 10 mM）作为洗脱剂。分离过程通过洗脱水相进行，有机相作为固定相。从1.0 g粗提物中，在4.5小时内分离得到氧化槐果碱（170 mg）和苦参碱（600 mg），纯度高于98%。
