Ratiometric confocal Ca2+ measurements with visible wavelength indicators in d-amphetamine-treated central snail neuron.

1. The bursting firing of action potentials and cytosolic calcium content in d-amphetamine-treated RP4 neurons of the African snail, Achatina fulica Ferussac, were studied elelctrophysiologically and optopharmacologically. 2. d-Amphetamine elicited bursting of firing of action potentials in central RP4 neuron. The bursting firing of action potentials was blocked following extracellular application of ruthenium red, the calcium uptake and release inhibitor from mitochondria. 3. At the same neuron, image analysis using ratiometric measurement on calcium green 1 and Texas red dextran (70,000 MW) with laser-scanning confocal microscopy revealed that cytosolic calcium content was increased after d-amphetamine treatment. 4. Our results support the view that the bursting firing of action potentials elicited by d-amphetamine is associated with cytosolic calcium content.