Eriksson P S, Perfilieva E, Björk-Eriksson T, Alborn A M, Nordborg C, Peterson D A, Gage F H
Department of Clinical Neuroscience, Institute of Neurology, Sahlgrenska University Hospital, Göteborg, Sweden.
Nat Med. 1998 Nov;4(11):1313-7. doi: 10.1038/3305.
The genesis of new cells, including neurons, in the adult human brain has not yet been demonstrated. This study was undertaken to investigate whether neurogenesis occurs in the adult human brain, in regions previously identified as neurogenic in adult rodents and monkeys. Human brain tissue was obtained postmortem from patients who had been treated with the thymidine analog, bromodeoxyuridine (BrdU), that labels DNA during the S phase. Using immunofluorescent labeling for BrdU and for one of the neuronal markers, NeuN, calbindin or neuron specific enolase (NSE), we demonstrate that new neurons, as defined by these markers, are generated from dividing progenitor cells in the dentate gyrus of adult humans. Our results further indicate that the human hippocampus retains its ability to generate neurons throughout life.
在成人大脑中，包括神经元在内的新细胞的生成尚未得到证实。本研究旨在调查在成人大脑中，先前在成年啮齿动物和猴子中被确定为神经发生区域的地方是否会发生神经发生。人类脑组织是在患者死后从接受过胸腺嘧啶核苷类似物溴脱氧尿苷（BrdU）治疗的患者身上获取的，BrdU在S期标记DNA。通过对BrdU以及神经元标志物之一NeuN、钙结合蛋白或神经元特异性烯醇化酶（NSE）进行免疫荧光标记，我们证明，由这些标志物定义的新神经元是由成人大脑齿状回中分裂的祖细胞产生的。我们的结果进一步表明，人类海马体在一生中都保留着生成神经元的能力。
