de Caro G, Polidori C, Beltz T G, Johnson A K
Department of Pharmacological Sciences and Experimental Medicine, University of Camerino, Italy.
Peptides. 1998;19(8):1399-406. doi: 10.1016/s0196-9781(98)00099-0.
Bombesin (BN) injected to sodium depleted rats either centrally, particularly into the paraventricular nucleus (PVN) or peripherally by intraperitoneal (IP) route, exerts a potent inhibitory effect on the intake of 2% sodium chloride. To determine whether the area postrema (AP) and the lateral parabrachial nucleus (LPBN), which are known to be involved in the control of ingestive behavior, could be sites for the antinatiorexic activity of BN, we studied the effects of injections of this peptide into the LPBN or, by IP or fourth ventricular route, to surgically AP-lesioned rats. We observed that in sodium depleted rats: 1) injected into the LPBN at a dose of 50, but not of 25, ng per nucleus, BN significantly reduced the intake of 2% sodium chloride; 2) administered either intraperitoneally or into the fourth brain ventricle, BN induced a potent antinatriorexic effect in AP-sham lesioned animals; and 3) in the same experimental conditions, surgical ablation of the AP did not reduce the antinatriorexic effect of the peptide. These data indicate that the LPBN may be, with the PVN, a site for the BN-ergic inhibitory control of salt appetite and that the AP, which has been implicated in BN-induced anorexia, does not play a role in the antinatriorexic effect of this peptide.
向缺钠大鼠中枢注射蛙皮素（BN），尤其是注入室旁核（PVN），或经腹腔（IP）途径外周注射，对2%氯化钠的摄入量有显著抑制作用。为了确定已知参与摄食行为控制的最后区（AP）和外侧臂旁核（LPBN）是否可能是BN抗钠食欲活性的作用部位，我们研究了将该肽注入LPBN，或经IP或第四脑室途径注射到手术切除AP的大鼠体内的效果。我们观察到，在缺钠大鼠中：1）以每核50 ng而非25 ng的剂量注入LPBN时，BN显著降低了2%氯化钠的摄入量；2）经腹腔或第四脑室给药时，BN在假手术切除AP的动物中诱导出显著的抗钠食欲作用；3）在相同实验条件下，手术切除AP并未降低该肽的抗钠食欲作用。这些数据表明，LPBN可能与PVN一样，是BN能抑制盐食欲的作用部位，且AP虽与BN诱导的厌食有关，但在该肽的抗钠食欲作用中不起作用。
