Li L, Moroz T P, Garden R W, Floyd P D, Weiss K R, Sweedler J V
Department of Chemistry and Beckman Institute, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Peptides. 1998;19(8):1425-33. doi: 10.1016/s0196-9781(98)00094-1.
The major ganglionic connectives in Aplysia are assayed to determine putative neuropeptides. Matrix-assisted laser desorption/ionization mass spectrometry allows direct measurement of peptides in a nerve. Many previously characterized peptides are observed, including APGWamide, buccalins, small cardioactive peptides, and egg-laying hormone. Several unreported peptides are detected in specific nerves, suggesting they may have important physiological roles. Furthermore, novel processing products of the L5-67 precursor peptide and the APGWamide/cerebral peptide 1 prohormone are strongly suggested, and their interganglionic transport demonstrated.
对海兔的主要神经节连接进行分析，以确定假定的神经肽。基质辅助激光解吸/电离质谱法可直接测量神经中的肽。观察到许多先前已鉴定的肽，包括APGW酰胺、颊肽、小的心脏活性肽和产卵激素。在特定神经中检测到几种未报道的肽，这表明它们可能具有重要的生理作用。此外，强烈提示了L5 - 67前体肽和APGW酰胺/脑肽1前激素的新加工产物，并证明了它们在神经节间的运输。
